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Characterization of a yeast artificial chromosome contig spanning the Huntington's disease gene candidate region

Nature Genetics volume 1, pages 180–187 (1992)Cite this article

Abstract

The Huntington's disease (HD) gene has been localized by recombination events to a region covering 2.2 megabases (Mb) DNA within chromosome 4p16.3. We have screened three yeast artificial chromosome (YAC) libraries in order to isolate and characterize 44 YAC clones mapping to this region. Approximately 50% of the YACs were chimaeric. Unstable YACs were identified across the whole region, but were particularly prevalent around the D4S183 and D4S43 loci. The YACs have been assembled into a contig extending from D4S126 to D4S98 covering roughly 2 Mb DNA, except for a gap of about 250 kilobases (kb). The establishment of a YAC contig which spans the region most likely to contain the HD mutation is an essential step in the isolation of the HD gene.

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Authors and Affiliations

  1. Genome Analysis Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK

    G. P. Bates, H. Hummerich, S. Baxendale, M. Ross & H. Lehrach

  2. Department of Internal Medicine and Human Genetics, and the Howard Hughes Medical Institute, University of Michigan, Ann Arbor, Michigan, 48109, USA

    J. Valdes, D. Tagle & F. Collins

  3. Centre D'Etude du Polymorphisme Humain, 27 rue Juliette Dodu, F-75010, Paris, France

    D. L. Le Paslier & D. Cohen

  4. Human Genetics Laboratory (ICRF), Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, UK

    A. P. Monaco

  5. Neurogenetics Laboratory, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts, 02114, USA

    M. E. MacDonald & J. F. Gusella

  6. Department of Biological Chemistry, College of Medicine, University of California, Irvine, California, 92717, USA

    M. Altherr & J. J. Wasmuth

  7. Department of Genetics, Washington University School of Medicine, St. Louis, Missouri, 63110, USA

    B. H. Brownstein

  8. Division of Medical and Molecular Genetics, Guy's Hospital, London, SE1 9RT, UK

    D. Bentley

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  1. G. P. Bates
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Bates, G., Valdes, J., Hummerich, H. et al. Characterization of a yeast artificial chromosome contig spanning the Huntington's disease gene candidate region. Nat Genet 1, 180–187 (1992). https://doi.org/10.1038/ng0692-180

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