Abstract
Successful adoptive T-cell therapy has been demonstrated in viral disease1,2 and selected forms of cancer3. However, it is limited by the difficulty to efficiently isolate and amplify autologous tumor-reactive T-cell clones. Tetramers of major histocompatibility complex (MHC) class I and peptide have facilitated the characterization of CD8+ T cells specific for tumor-associated antigens4,5. However, for adoptive T-cell therapy, MHC-tetramers have limitations: they require knowledge of tumor antigens, which is often not available; they select T cells with a single specificity, thereby posing risk for selection of tumor escape variants; they do not select for function, so that T cells may be anergic when isolated from cancer patients; and they do not allow the isolation of CD4+ T cells that can be essential for tumor rejection6. Because interferon (IFN)-γ is essential for tumor rejection7,8, we isolated live T cells based on their IFN-γ production9. IFN-γ secreted by previously activated T cells is retained on the cell surface, allowing their specific isolation and expansion10. We show here that IFN-γ+ but not IFN-γ− T cells from tumor-immunized mice are cytolytic and mediate tumor rejection upon adoptive transfer. Importantly, tumor-specific T cells can be enriched from lymphocytes infiltrating human renal cell carcinoma by the IFN-γ capture assay.
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Acknowledgements
This work was supported by grants through the Deutsche Forschungsgemeinschaft (SFB 506 and HO1596), the Deutsche Krebshilfe, Mildred-Scheel-Stiftung, e.V. and the Bundesministerium für Bildung und Forschung.
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Becker, C., Pohla, H., Frankenberger, B. et al. Adoptive tumor therapy with T lymphocytes enriched through an IFN-γ capture assay. Nat Med 7, 1159–1162 (2001). https://doi.org/10.1038/nm1001-1159
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DOI: https://doi.org/10.1038/nm1001-1159
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