Elsevier

Scientia Horticulturae

Volume 51, Issues 3–4, September 1992, Pages 187-195
Scientia Horticulturae

Involvement of rooting factors and free IAA in the rootability of citrus species stem cuttings

https://doi.org/10.1016/0304-4238(92)90118-VGet rights and content

Abstract

Two-year-old trees of cultivar ‘Rangpur’ lime (Citrus limonia Osb.) and of cultivar ‘Oroblanco’, a triploid pummelo-grapefruit hybrid (Citrus grandis Osb. × Citrus paradisi Macf.), which had not reached flowering stage, were grown under greenhouse conditions. Cuttings from the last vigorous vegetative flush were taken from each species for rooting experiments. Callus formation and percentage of rooting were determined after 19 and 36 days. Endogenous indole-3-acetic acid (IAA) content and avocado rooting promoter (ARP)-like activity were determined in leaves and bark (cortex) of the lower end of the cutting on day of excision and 19 days later. Rooting of the easy-to-root ‘Rangpur’ lime reached 77% after 19 days and 100% after 36 days. At those times the difficult-to-root ‘Oroblanco’ did not root at all or reached 12% rooting, respectively. At Day 19 the level of free IAA in the bark of ‘Rangpur’ lime was 3.5 times higher than that at Day 0, and 3.6 times higher than in the ‘Oroblanco’ bark on the same day. ARP was found in both species at excision day and after 19 days using gas-chromatography analysis. The ARP activity on the day of excision was only slightly higher in the leaves and bark of ‘Rangpur’ lime than in ‘Oroblanco’, but after 19 days ARP-like activity rose approximately 45% in the basal bark of ‘Rangpur’ lime with no such increase in its leaves or in the leaves and bark of ‘Oroblanco’. The differences in IAA level and ARP-like activity in the two citrus cultivars appear to be correlated with their ease of rooting, but it is not possible to tell whether the increase in IAA and ARP over the rooting period is the cause or result of root initiation.

References (26)

  • G.R. Beck et al.

    The response of poinsettia cultivars to auxins in root promotion of stem cuttings

    Scientia Hortic.

    (1974)
  • J.D. Cohen

    Convenient apparatus for the generation of small amounts of diazomethane

    J. Chromatogr.

    (1984)
  • D. Becker et al.

    Avocado rooting promoter (ARP) detection in Laurus nobilis L. and Magnifera indica L. based on gas chromatography

    Scientia Hortic.

    (1991)
  • E.N. Ericksen

    Root formation in pea cuttings II. The influence of indole-3-acetic acid at different development stages

    Physiol. Plant.

    (1974)
  • T. Gaspar et al.

    Auxin metabolism during adventitious rooting

  • C.J. Gorter

    Auxin-synergists in the rooting of cuttings

    Physiol. Plant.

    (1969)
  • B.E. Haissig

    Influence of indole-3-acetic acid on adventitious root primordia of brittle willow

    Planta

    (1970)
  • H.T. Hartmann et al.

    Plants propagation. Principles and Practices

  • T. Hemberg

    The relation between the occurrence of auxin and rooting of hypocotyls in Phaseolus vulgaris L.

    Physiol. Plant.

    (1954)
  • C.E. Hess

    Rooting co-factors, identification and functions

  • B.C. Jarvis

    Endogenous control of adventitious rooting in non-woody cuttings

  • M.C. Mato et al.

    Changes in auxin protectors and IAA oxidases during rooting of chesnut shoots in vitro

    Physiol. Plant.

    (1986)
  • S. Mohammed et al.

    Root formation in pea cuttings. IV. Further studies on the influence of indole-3-acetic acid at different development stages

    Physiol. Plant.

    (1974)
  • Cited by (0)

    View full text