Effect of pulsed magnetic fields on human umbilical endothelial vein cells

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Abstract

The long-term goal of this program was to examine the efficacy of weak electromagnetic fields as an adjunct therapy to facilitate wound repair. The experiments reported here were directed to the question of whether pulsed magnetic fields (PMFs) could facilitate endothelial cell migration following induction of a wound. These studies were based on a report that weak PMFs facilitated cell migration in a wounded confluent monolayer culture of human umbilical vein cells. Experiments reported in this paper were an attempt to reproduce and extend these observations. Our data support the original observations. Further, we examined several hypotheses in an attempt to clarify the mechanism of interaction between the applied electromagnetic fields and cells. The data do not support the hypothesis that PMFs are acting in a manner analogous to endothelial cell growth factor, a normal component of the growth medium, or that PMFs applied at the time of cell transfer enhance DNA replication. We have found that PMFs are more effective in accelerating migration if they induce an electric field that is perpendicular rather than parallel to the sides of the wound.

References (19)

  • R. Goodman et al.

    Biochim. Biophys. Acta

    (1989)
  • R. Goodman et al.

    Bioelectrochem. Bioenerg.

    (1992)
  • P.H. Delport et al.

    Bioelectrochem. Bioenerg.

    (1985)
  • E.M. Goodman et al.

    FEBS Lett.

    (1986)
  • M.T. Marron et al.

    FEBS Lett.

    (1988)
  • C.A.L. Bassett

    Orthop. Clin. N. Am.

    (1984)
  • T. Sorger et al.

    Anal. Biochem.

    (1983)
  • A.R. Liboff et al.

    Science

    (1984)
  • K. Takahashi et al.

    Experientia

    (1986)
There are more references available in the full text version of this article.

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