Binding properties of duodenal 1,25-dihydroxyvitamin D3 receptors as affected by phosphorus depletion in lactating goats

https://doi.org/10.1016/0300-9629(90)90668-IGet rights and content

Abstract

  • 1.

    1. Capacity and affinity of duodenal 1,25(OH)2D3 receptors were measured in P depleted goats and in control animals kept on an adequate P supply. Plasma concentrations of Pi Ca and vitamin D3 metabolites and activity of plasma alkaline phosphatase were measured to characterize the effects of P depletion.

  • 2.

    2. During P depletion plasma Pi concentrations decreased significantly whereas plasma Ca and alkaline phosphatase activity increased. No changes were recorded for plasma vitamin D3 250HD3 and 1,25(OH)2D3 concentrations.

  • 3.

    3. P depletion resulted in a significant decrease of the equilibrium dissociation constant Kd of duodenal 1,25(OH)2D3 receptors without affecting the maximum binding capacity.

References (27)

  • M.J Favus et al.

    Evidence for in vivo upregulation of the intestinal vitamin D receptor during dietary calcium restriction in the rat

    J. clin. Invest.

    (1988)
  • J Fox et al.

    Effect of low phosphorus diets on intestinal calcium absorption and the concentration of calcium binding protein in intact and parathyroidectomised pigs

    J. Endocr.

    (1978)
  • M.R Hughes et al.

    Regulation of serum 1,25-dihydroxyvitamin D3 by calcium and phosphate in the rat

    Science

    (1975)
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      The final pellet was resuspended in the second preparation buffer and stored at −80 °C. Whole membrane fractions were prepared as already described (Schröder et al., 1990) with slight modifications as described elsewhere (Muscher et al., 2008) for caprine kidney cortex. Whole cell lysates were prepared by homogenising the tissue in preparation buffer containing 50 mmol/L HEPES, 4 mmol/L EGTA, 10 mmol/L EDTA, 0.1% Triton X-100, 100 mmol/L β-glycerophosphate, 15 mmol/L pyrophosphate, 15 mmol/L sodium orthovanadate, 5 mmol/L sodium fluoride, protease and phosphatase inhibitors (Complete mini, Roche, Mannheim, Germany), pH 7.4.

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