Elsevier

Gene

Volume 93, Issue 1, 1 September 1990, Pages 79-84
Gene

Phosphate control of pabS gene transcription during candicidin biosynthesis

https://doi.org/10.1016/0378-1119(90)90139-IGet rights and content

Abstract

The pabS gene of Streptomyces griseus IMRU3570 encodes the enzyme p-aminobenzoic acid synthase, which synthesizes p-aminobenzoic acid (PABA), a precursor of the antibiotic candicidin (Cd). The pabS transcript reached a peak at 12 h of incubation in batch cultures, preceding the formation of PABA synthase and the antibiotic itself. A decay of the pabS transcript was observed with an apparent half-life of 35 min. Inorganic phosphate (Pi; 7.5 mM) reduced the synthesis of the pabS transcript by 90–95%, and consequently the formation of PABA synthase and Cd. Thirty min after addition of 7.5 mM Pi, the cells synthesized only about 15% as much pabS transcript compared to control cultures. However, Pi stimulated two- to threefold total RNA synthesis. The 1.7-kb pabS transcript shown by Northern hybridization was greatly reduced in amount in cells grown in 7.5 mM phosphate. Pi-deregulated mutants, described previously, were impaired in the transcriptional control exerted by Pi. It is concluded that Pi control of PABA synthase and Cd biosynthesis is exerted by repression of formation of the pabS mRNA.

Reference (30)

  • GilJ.A. et al.

    Characterization and regulation of p-aminobenzoic acid synthase from Streptomyces griseus

    J. Gen. Microbiol.

    (1985)
  • HopwoodD.A. et al.
  • HorinouchiS. et al.

    afsB stimulates transcription of the actinorhodin biosynthetic pathway in Streptomyces coelicolor A3(2) and Streptomyces lividans

    Mol. Gen. Genet.

    (1989)
  • JonesG.H.

    Regulation of phenoxazinone synthase expression in Streptomyces antibioticus

    J. Bacteriol.

    (1985)
  • KimuraS. et al.

    Regulation of the phosphate regulom of Escherichia coli: Characterization of the promoter of the pstS gene

    Mol. Gen. Genet.

    (1989)
  • Cited by (29)

    • chapter 10 Enzymology of the Polyenes Pimaricin and Candicidin Biosynthesis

      2009, Methods in Enzymology
      Citation Excerpt :

      Characterization of pimaricin production in phoP-disrupted mutants and in phoR-phoP deletion mutants revealed that both mutants showed reduced sensitivity to phosphate control and an important increase in antifungal production (up to 80%) (Mendes et al., 2007b). Transcriptional control by phosphate of genes involved in polyene macrolide antibiotic biosynthesis has also been reported for candicidin (Asturias et al., 1990), suggesting that production of other polyenes might be increased by manipulation of this regulatory locus. The presence of γ-butyrolactones in different species of Streptomyces and their role in triggering secondary metabolite biosynthesis is well documented (Horinouchi and Beppu, 1992).

    • Regulation of secondary metabolites of actinobacteria

      2017, Biology and Biotechnology of Actinobacteria
    View all citing articles on Scopus
    View full text