Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa

doi:10.1016/0378-1119(94)90237-2

Gene

Volume 148, Issue 1, 11 October 1994, Pages 81-86

https://doi.org/10.1016/0378-1119(94)90237-2 Get rights and content

Abstract

The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance ofpMB1(ColE1)-based cloning vectors in Pseudomonas, was determined. This fragment encodes a putative origin of replication (ori), a replication-controlling protein, and the C terminus of the Tn3 β-lactamase-encoding gene. Improved versions of the broad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.

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^☆: Requests for plasmids should be sent to Dr. H.P. Schweizer, Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta T2N 4N1, Canada. Tel. (403) 220-8302; Fax (403) 283-8814;

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Short communicationConstruction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa☆

Abstract

Gene

Cell

Plasmid

Cell

FEMS Microbiol. Lett.

Gene

J. Mol. Biol.

Gene

Gene

Gene

J. Mol. Biol.

Gene

Gene

Construction of broad-host-range cosmid cloning vectors: identification of genes necessary fur growth of Methylobacterium organophilum on methanol

J. Bacteriol.

Host:vector systems for gene cloning in Pseudomonus

Curr. Top. Microbiol. Immunol.

7-deaza-2′-deoxyguanosine-5′-triphosphate: enhanced resolution in M13 dideoxy sequencing

BioTechniques

Relationship of group P1 plasmids revealed by heteroduplex experiments: RPl, RP4, R68, and RK2 are identical

J. Gen. Microbiol.

Short communication
Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa☆