Interaction between dermatan sulphate chains I. Affinity chromatography of copolymeric galactos-aminoglycans on dermatan sulphate-substituted agarose

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Abstract

  • 1.

    (1) Binding of copolymeric as well as homopolymeric galactosaminoglycans to dermatan sulphate-substituted gels has been demonstrated. Material bound in the presence of 0.15 M NaCl was eluted with either 1 M urea, 0.5 M guanadine · HCl or 0.5 M NaCl. Homopolymeric galactosaminoglycans were also displaced by 0.5 M sodium acetate. The interaction was not dependent on divalent cations.

  • 2.

    (2) Dermatan sulphate has been fractionated into aggregating and non-aggregating species by gel chromatography in the presence of 0.5 M sodium acetate. In the presence of 0.1 M sodium acetate or 0.5 M guanidine · HCl no aggregation was observed.

  • 3.

    (3) Crosslinks formed during periodate oxidation at physiological ionic strength have been ascribed to chain-chain interaction.

  • 4.

    (4) Chondroitin 4-sulphate, heparan sulphate and heparin also showed interaction with gels substituted with copolymeric galactosaminoglycans, while chondroitin 6-sulphate, hyaluronate and keratan sulphate did not.

  • 5.

    (5) Binding of copolymeric galactosaminoglycan chains to dermatan sulphate- or chondroitin sulphate-substituted gels was most pronounced when the copolymeric chains contained similar proportions of L-iduronic and D-glucuronic acid.

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