Prostaglandins and steroidogenesis in isolated bovine adrenal cells: Effects of acth, prostaglandin-synthesis inhibitors, prostaglandins and prostaglandin analogs

doi:10.1016/0303-7207(81)90090-3

Molecular and Cellular Endocrinology

Volume 22, Issue 2, May 1981, Pages 179-193

https://doi.org/10.1016/0303-7207(81)90090-3 Get rights and content

Abstract

In bovine adrenal cortex cells, dispersed without preferential loss of cells, we investigated (1) whether endogenous prostaglandins (PGs) are involved in ACTH-induced adrenal steroido-genesis, arid (2) the steroidogenic effects of PGs and PG analogs.

Free cells produced considerable amounts of PGE₂, whereas only minute quantities of PGF_2α and PGA₁ were synthesized. PGE₂ synthesis, however, was not significantly increased when ACTH elicited a steroidogenic response in free cells. High concentrations of PG-synthesis inhibitors such as indomethacin affected both PG synthesis and steroidogenesis, whereas intermediate concentrations (10⁻⁶ M) inhibited production of both PGE₂ and aldosterone even after cAMP and cortisol response to ACTH had returned to normal values. It is concluded that endogenous PGE₂ is not a link in the acute mechanism of action of trophic hormones in which cAMP is involved.

Of the prostanoid structures, PGs of the E series were the most potent stimulating agents of cortisol production, although less active than ACTH. On the other hand, PGA₁ induced an ACTH-like aldosterone synthesis. PGE₂ was less active and other prostanoid structures were without effect on aldosterone production. It is suggested that in pathological circumstances, PGA₁ regulates aldosterone production and PGE₂ increases both aldosterone and cortisol production.

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Prostaglandins and steroidogenesis in isolated bovine adrenal cells: Effects of acth, prostaglandin-synthesis inhibitors, prostaglandins and prostaglandin analogs

Abstract

Prostaglandins

J. Lipid Res.

Prostaglandins

Prostaglandins

Prostaglandins

Mol. Cell. Endocrinol.

Human Pathol.

FEBS Lett.

Mol. Cell. Endocrinol.

Prostaglandins

Anal. Biochem.

Endocrinology

Life Sci.

Prostaglandins

Endocrinology

Circulat. Res.

Am. J. Med.

Adv-Prostagland. Thromb. Res.

Adv. Prost. Thromb. Res.

Am. J. Med.

Acta Endocrinol.

J. Endocrinol.

Adv. Prost. Thromb. Res.

Prostaglandins

Adv. Cyclic Nucleot. Res.

Adv. Prostagland. Thromb. Res.

Endocrinology

Helv. Chim. Acta

Histochemistry

Steroids