Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology
Regular paperCharge isomers of urinary bikunin (trypsin inhibitor)
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Enzymatic synthesis of hyaluronan hybrid urinary trypsin inhibitor
2015, Carbohydrate ResearchCitation Excerpt :The core protein of UTI consists of 143 amino acids and has a single low-sulfated chondroitin 4-sulfate (Ch4S) chain covalently linked to the residue Ser-10 as a glycosaminoglycan (GAG) moiety through a GlcUAβ1-3Galβ1-3Galβ1-4Xylβ1-Ser (GlcUA-Gal-Gal-Xyl-Ser) or GlcUA-Gal(4-sulfate)-Gal-Xyl-Ser linkage region.5–7 Clustered sulfated units (Ch4S disaccharides) are located at the reducing terminus of the GAG chain linked to the linkage region, while clustered non-sulfated units (chondroitin disaccharides, Ch) are located at the non-reducing terminus, and the number of both units can vary in UTI.8–10 UTI has been used as an anti-inflammatory medicine for pancreatitis, threatened premature delivery, and other conditions based on the protease inhibitor activity of its core protein.11
Sulfation of the bikunin chondroitin sulfate chain determines heavy chain-hyaluronan complex formation
2013, Journal of Biological ChemistryCitation Excerpt :These data agree with fraction 2 analyzed in this study that eluted at salt concentrations higher than 0.5 m NaCl. Approximately 30 isomers of urinary bikunin have been isolated with differences in the degree of CS chain sulfation and length (23, 47). Separation of urinary bikunin in this study using anion exchange chromatography and subsequent analysis of the disaccharide composition of the CS chains supported the presence of isomers.
Diversity in the degree of sulfation and chain length of the glycosaminoglycan moiety of urinary trypsin inhibitor isomers
2007, Biochimica et Biophysica Acta - General SubjectsExcretion of bikunin and its fragments in the urine of patients with renal stones
2001, Journal of UrologyBikunin - Not just a plasma proteinase inhibitor
2000, International Journal of Biochemistry and Cell Biology