Effect of phospholipase A2 on Ca2+-stimulated adenosine triphosphatase activity in microsomal fraction of rat submandibular gland

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Abstract

  • 1.

    1. The effect of phospholipase A2 on the Ca2+-ATPase (EC 3.6.1.3) activity in the microsomal fraction of rat submandibular gland was kinetically studied in vitro.

  • 2.

    2. The Ca2+-ATPase activity was significantly increased by the treatment with phospholipase A2 in the presence of bovine serum albumin as a scavenger for hydrolyzed products.

  • 3.

    3. When the microsomal fraction was incubated with phospholipase A2 in the absence of bovine serum albumin, the Ca2+-ATPase activity was not altered.

  • 4.

    4. The Vmax and Km values for both ATP and Ca2+ were increased by the phospholipase A2 treatment, respectively.

  • 5.

    5. These results indicated that the activation of Ca2+-ATPase by the phospholipase A2 treatment is due to the increase of Vmax.

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    • Cited by (1)

    • Effect of dietary essential fatty acid deficiency on Ca<sup>2+</sup>-atpase activity in microsomal fraction of rat submandibular gland

      1991, International Journal of Biochemistry
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