Biochimica et Biophysica Acta (BBA) - Enzymology
Purification, general properties and two other catalytic activities of α-ketoglutarate:glyoxylate carboligase of Mycobacterium phlei
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Cited by (4)
Influence of allosteric regulators on individual steps in the reaction catalyzed by mycobacterium tuberculosis 2-hydroxy-3-oxoadipate synthase
2013, Journal of Biological ChemistryFunctional plasticity and allosteric regulation of α-ketoglutarate decarboxylase in central mycobacterial metabolism
2011, Chemistry and BiologyCitation Excerpt :To our knowledge, no carboligase or decarboxylase activities had been previously reported for the E1o component of the E. coli KDH complex. Nevertheless, our findings are consistent with the biochemical detection of these different ThDP-dependent activities in cell extracts from a number of bacterial species, including E. coli and mycobacteria (Yamasaki and Moriyama, 1970, 1971), and can also account for early reports describing multiple activities associated with bacterial and mammalian KDH complexes (Bunik and Fernie, 2009; O'Fallon and Brosemer, 1977; Schlossberg et al., 1970). The FHA domain-containing protein GarA has been recently identified as a phospho-dependent regulator of metabolic enzymes (including KGD) involved in glutamate synthesis under control of the Ser/Thr protein kinase PknG in both corynebacteria and mycobacteria (Niebisch et al., 2006; Bott, 2007; O'Hare et al., 2008; Nott et al., 2009).
Activity-Based Metabolomic Profiling of Enzymatic Function: Identification of Rv1248c as a Mycobacterial 2-Hydroxy-3-oxoadipate Synthase
2010, Chemistry and BiologyCitation Excerpt :HOAS activity (EC 2.2.1.5) has been described as a side reaction of the E1 component of KDH complex that occurs in the absence of a functional KDH complex (O'Fallon and Brosemer, 1977; Schlossberg et al., 1970). Before DNA sequencing and cloning were widespread, HOA and HLA were detected in animals, plants, fungi, and bacteria (O'Fallon and Brosemer, 1977; Saito et al., 1971; Schlossberg et al., 1970; Yamasaki and Moriyama, 1970a, 1971), where it was speculated that they might be involved in GLX detoxification or synthesis of secondary metabolites. Moreover, early studies of protein extracts of Mycobacterium phlei identified the synthesis of HOA and its conversion to HLA, which was dependent on α-KG, GLX, TDP, and Mg2+, though the gene or protein encoding this activity was never assigned (Yamasaki and Moriyama, 1970b, 1971).
Isoelectric points and molecular weights of proteins. A table
1976, Journal of Chromatography A