Induction of the nuclear protein cyclin in serum-stimulated quiescent 3T3 cells is independent of DNA synthesis

doi:10.1016/0014-4827(85)90552-X

Experimental Cell Research

Volume 156, Issue 2, February 1985, Pages 455-461

https://doi.org/10.1016/0014-4827(85)90552-X Get rights and content

Abstract

Inhibition of DNA synthesis and cell proliferation of mouse 3T3 cells by aphidicolin did not affect the expression of cyclin, a nuclear protein whose synthesis correlates with cell proliferation, as determined by quantitative two-dimensional gel electrophoresis analysis. Serum stimulation of quiescent 3T3 cells revealed that cyclin synthesis increases shortly before DNA synthesis. Inhibition of DNA synthesis by aphidicolin in serum-stimulated quiescent cells did not affect the increase of cyclin following stimulation. These results demonstrate that cyclin synthesis is not coupled to DNA synthesis and that it is one of the latest events before DNA replication.

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Induction of the nuclear protein cyclin in serum-stimulated quiescent 3T3 cells is independent of DNA synthesis

Abstract

Exp cell res

Cell biol int rep

Exp cell res

FEBS lett

FEBS lett

Biochem biophys res commun

Exp cell res

Biochem biophys res commun

J mol biol

J biol chem

Adv immunol

Carcinogenesis