Elsevier

Analytical Biochemistry

Volume 143, Issue 2, December 1984, Pages 308-315
Analytical Biochemistry

Oxidative determination of 14C-labeled 2-oxo acids

https://doi.org/10.1016/0003-2697(84)90668-7Get rights and content

Abstract

A simple and rapid assay for the determination of 1-14C- or U-14C-labeled 2-oxo acids is described. It is based on the selective and complete oxidation of the carboxyl group to 14CO2. Preceding purification procedures are not necessary. In rat hindlimb perfusion studies, the procedure was used to develop an indirect method for the estimation of the intracellular dilution of [1-14C]pyruvate and to determine the relationship between the transamination and decarboxylation rates of leucine in the perfused tissue by the use of tracer doses of l-[1-14C]leucine.

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