Effects of heavy ions on inactivation and DNA double strand breaks in Deinococcus radiodurans R1

doi:10.1016/0273-1177(94)90470-7

Advances in Space Research

Volume 14, Issue 10, October 1994, Pages 213-216

https://doi.org/10.1016/0273-1177(94)90470-7 Get rights and content

Abstract

Inactivation and double strand break (dsb) induction after heavy ion irradiation were studied in stationary phase cells of the highly radiation resistant bacterium Deinococcus radiodurans R1.

There is evidence that the radiation sensitivity of this bacterium is nearly independent on energy in the range of up to 15 MeV/u for lighter ions (Ar). The responses to dsb induction for charged particles show direct relationship between increasing radiation dose and residual intact DNA.

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The results obtained from this experiment led to the conclusion that the spores (1 μm diameter) on the outer areas were not affected by the high-energy particles of the galactic cosmic radiations; instead, a “bystander effect” was observed in which the effect of charged particles traversed from the outer layer of spores to the surrounding spores, where a biological effect was observed. Additionally, DNA is more prone to be damaged by radiation through the formation of double-stranded breaks that ultimately result in mutations, as observed in E. coli, B. subtilis, and Deinococcus radiodurans (Micke et al., 1994; Schafer et al., 1994; Zimmermann et al., 1994). Microorganisms possess several mechanisms to repair DNA, either by homologous recombination or non-homologous end joining (NHEJ).
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Effects of heavy ions on inactivation and DNA double strand breaks in Deinococcus radiodurans R1

Abstract

Methods in Enzymology

Biochem. Biophys. Res. Commun.

The survival of Micrococcus radiodurans irradiated at high LET and the effect of acridine orange

Int. J. Radiat. Biol.

Inactivation of a radioresistant vegetative bacterium Micrococcus radiodurans by charged particles

IPCR Cyclotron Prog. Rep.

LET effects in Micrococcus radiodurans irradiated with alpha particles in the liquid phase

Agr. Biol. Chem.