Biosynthesis of porcine kidney D-amino acid oxidase1

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The biosynthesis of a porcine kidney peroxisomal enzyme, D-amino acid oxidase (EC 1.4.3.3., DAO), was investigated. Pig kidney mRNA as well as free and membrane-bound polysomes were used to investigate in vitro protein synthesis using a rabbit reticulocyte lysate. mRNA and free polysomes, but not membrane-bound polysomes, directed the synthesis of DAO. To examine the in vivo synthesis of the enzyme, a pig kidney cell line (LLC-PK1) was biosynthetically labelled. Both the in vitro and in vivo synthesized DAO had the same molecular weight, 38,000, as that of the purified enzyme. These results indicate strongly that DAO is synthesized on free ribosomes and transferred to the interior of peroxisomes without any proteolytic modification.

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1

This study was supported in part by a Research Grant for Cardiovascular Diseases (59C-8) from the Ministry of Health and Welfare of Japan, and Grants-in-Aid for Scientific Research (No.61570151) and for Special Project Research (No.61132005) from the Ministry of Education, Science and Culture of Japan.

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