Elsevier

Carbohydrate Research

Volume 14, Issue 3, September 1970, Pages 287-296
Carbohydrate Research

Cross-linked polyacrylamide derivatives (enzacryls) as water-insoluble carriers of amylolytic enzymes

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Abstract

Methods are described for the water-insolubilisation of alpha- and beta-amylase by chemical coupling to two cross-linked copolymers of acrylamide. For one copolymer, coupling was effected either by diazo or isothiocyanato groups, and for the other, acid azide groups were employed. Active derivatives of alpha-amylase were prepared by diazo, isothiocyanato, and acid azide coupling, whereas active derivatives of beta-amylase could be prepared only by diazo and isothiocyanato coupling. The stabilities of the active, copolyacrylamide-bound preparations of amylase were comparable to those of the corresponding, cellulose-bound enzymes. In the case of alpha-amylase, the copolyacrylamide derivatives were more stable than the free enzyme in solution.

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