Elsevier

FEBS Letters

Volume 283, Issue 1, 20 May 1991, Pages 145-149
FEBS Letters

Identification and immunohistochemical mapping of GABAA receptor subtypes containing the δ-subunit in rat brain

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Abstract

Synaptic inhibition in brain is mainly mediated via GABAA receptors which display a striking structural heterogeneity. A novel type of GABAA receptor subunit, the δ-subunit, has recently been described based on molecular cloning of its cDNA [1]. To identify the prevalence and distrubution of GABAA receptors which contain the δ-subunit protein in situ, polyclonal site-directed antisera were developed against three synthetic peptides derived form the rat δ-subunit cDNA-sequence. All antisera specifically recognized a 54 kDa protein in GABAA receptor preparation. Nearly 30% of the GABAA receptors contained the δ-subunit immunoreactivity and displayed high affinity GABA and high affinity benzodiazepine binding sites as shown by immunoprecipitation. Receptors which contain the δ-subunit were immunohistochemically shown to be restricted to a few brain areas such as the cerebellum, thalamus and dentate gyrus of the hippocampal formation. Thus, those neurons which express GABAA receptors with a δ-subunit have now been visualized and made accessible for a functional analysis of this GABAA receptors subtype in situ.

Keywords

GABAA receptor
δ-Subunit
Antipeptide antisera
Western blotting
Immunoprecipitation
Immunohistochemistry

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