Elsevier

FEBS Letters

Volume 263, Issue 1, 9 April 1990, Pages 137-141
FEBS Letters

Research letters
Effect of heparin on proliferation and signalling in BC3H-1 muscle cells: Evidence for specific binding sites

https://doi.org/10.1016/0014-5793(90)80723-VGet rights and content
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Abstract

We studied binding and growth inhibitory properties of different glycosaminoglycans in growing and differentiated BC3H-1 muscle cells. Heparin (10 μgml) and heparan sulfate (10 μgml) significantly inhibited DNA synthesis in growing and differentiated cells, as monitored by [3H] thymidine incorporation. Binding of heparin to BC3H-1 cells was specific and time-dependent. Heparan sulfate was the only glycosaminoglycan able to displace [3H]heparin (IC50, 3.2 × 10−7 M), although it was 10-fold less effective than heparin itself (IC50 3.6 × 1O−8 M). Scatchard analysis revealed the existence of high-affinity hepariy binding sites (Kd, 5 × 10−8 M). Furthermore, heparin inhibited serum-induced stimulation of inositol lipid turnover. Taken together, these results indicate that heparin inhibits BC3H-1 cell growth by interacting with the cell surface, possibly disrupting the flow of growth factor-related mitogenic signalling.

Keywords

Heparin
Cell growth
Inositol lipid
Cell signalling

Abbreviations

EGF, epidermal growth factor
PDGF, plateletderived growth factor
IGF-1, insulin-like growth factor-1
ECGF, endothelial cell growth factor
a-FGF, acidic fibroblast growth factor
GAGs, glycosaminoglycans
VLMW-heparin, very low molecular weight heparin
BSA, bovine serum albumin
PBS, phosphate-buffered saline

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