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Crystals of the esterase EstB were obtained at 277 K with the hanging-drop technique from polyethylene glycol 4000 solutions containing 2-propanol at pH 7.5. The crystals belong to the trigonal space group P3121 (or P3221) with cell dimensions a = b = 82.9 and c = 193.4 Å (at 100 K). The crystals diffract beyond a resolution of 2.0 Å.
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