Crystallization and preliminary X-ray analysis of pyruvate kinase type I from Escherichia coli

Crystals of the fructose-l,6-bisphosphate-dependent pyruvate kinase from Escherichia coli have been grown using the hanging-drop vapour-diffusion technique. The space group was found to be C222₁ with cell dimensions a = 76.8, b = 247.5, c = 132.6 Å. Diffraction data to 3.0 Å resolution have been recorded and the enzyme molecular symmetry analysed through inspection of the self-rotation function. The crystallized protein is in the allosterically inactive T state.