Purification, crystallization and X-ray analysis of crystals of pectate lyase A from Erwinia chrysanthemi

Pectate lyase A is secreted by Erwinia chrysanthemi and is a virulence factor for soft rot diseases in plants. Crystals of pectate lyase A were obtained by vapor-diffusion techniques in the presence of polyethylene glycol. The crystals belong to the monoclinic space group P2₁, with unit-cell parameters a = 48.96, b = 148.86, c = 78.61 Å, β = 97.32°. The crystals contain two protein molecules of 38 kDa per asymmetric unit and diffract to 2.4 Å using Cu Kα radiation.