Crystallization and preliminary X-ray diffraction analysis of the Saccharomyces cerevisiae Ran-­binding protein Mog1p

Mog1p binds the Ras-family GTPase Ran/Gsp1p, which has a central role in nucleocytoplasmic transport and cell-cycle progression. Overexpression of MOG1 is able to suppress temperature-sensitive gsp1 mutants in yeast; Δmog1 null mutants display temperature-sensitive defects in nuclear trafficking. Orthorhombic crystals of bacterially expressed Mog1p that diffract to beyond 2 Å resolution using synchrotron radiation have been obtained. The crystals have P2₁2₁2₁ symmetry, with unit-cell parameters a = 39.67, b = 51.96, c = 112.23 Å, a Matthews coefficient of 2.44 ų Da⁻¹, an estimated solvent content of 49.5% and one chain in the asymmetric unit.