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Convenient and optimized method for sample pre-treatment for the analysis of bile acids in biological matrices

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Summary

The analysis of bile acids in biological matrices, e.g. gall bladder bile and serum, depends on accurate and reproducible sample pre-treatment techniques. The present study compares published and commonly used protocols with a special focus on the analysis of serum bile acids. Since adsorption of bile acids to serum proteins has a dramatic impact on the recovery rate and the reproducibility of the method used, several deproteination procedures were compared. The combination of given protocols made it possible to develop a very sensitive and efficient sample pre-treatment which is well suited for all chromatographic methods for bile acid analysis.

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Abbreviations

HPLC:

high performance liquid chromatography

HPTLC:

high performance thin layer chromatography

CA:

cholate

LCA:

lithocholate

GCA:

glycocholate

TCA:

taurocholate

GCDCA:

glycochenodeoxycholate

TCDCA:

taurochenodeoxycholate

CDCA:

chenodeoxycholate

GDCA:

glycodeoxycholate

TDCA:

taurodeoxycholate

GLCA:

glycolithocholate

TLCA:

taurolithocholate

GUDCA:

glycoursodeoxycholate

TUDCA:

tauroursodeoxycholate

GHCA:

glycohyocholate

THCA:

taurohyocholate

GHDCA:

glycohyodeoxycholate

THDCA:

taurohyodeoxycholate

α-GMA:

α-glycomuricholate

α-TMA:

α-tauromuricholate

β-GMA:

β-glycomuricholate

β-TMA:

β-tauromuricholate

ω-GMA:

ω-glycomuricholate

ω-TMA:

ω-tauromuricholate

MeOH:

methanol

AcCN:

acetonitrile

CGH:

choloylglycine hydrolase

SPE:

solid phase extraction

BMC:

bromomethoxy coumarin

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Author notes

  1. C. I. Dax

    Present address: Department of Cell Biology, Harvard Medical School, MA 02115, Boston, Massachusetts, USA

Authors and Affiliations

  1. Universität des Saarlandes, 66123, Saarbrücken, Germany

    C. I. Dax

  2. S. Müllner, Aventis Research & Technologies GmbH & Co. KG, 65926, Frankfurt, Germany

    S. Müllner

Authors
  1. C. I. Dax
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  2. S. Müllner
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Dax, C.I., Müllner, S. Convenient and optimized method for sample pre-treatment for the analysis of bile acids in biological matrices. Chromatographia 48, 681–689 (1998). https://doi.org/10.1007/BF02467599

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  • DOI: https://doi.org/10.1007/BF02467599

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