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Selectivity studies on branched and linear alkyl bonded stationary phases in reversed-phase liquid chromatography

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Summary

Reversed-phase liquid chromatographic retention characteristics for the sixteen acyclic C1−C5 N-alkylbenzamide congeners were measured on various branched and linear, alkyl bonded hydrocarbon stationary phases. Retention factors, k′, were determined in acetonitrile-water mobile phase compositions on ethyl, n-octyl, n-dodecyl, n-octadecyl, 1-ethyladamantyl, 4-butyloctyl, and 2,4,4-trimethylpentyl stationary phases. Statistical analysis of the two main effects investigated — type of stationary phase and percentage of organic modifier (acetonitrile) in the mobile phase — described greater than ninety percent of the variability in the data for most of the comparisons. Selectivity effects due to variation in the mobile phase dominated the results.

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Author notes

  1. V. Rehak

    Present address: Analytical Department, Institute for Macromolecular Chemistry, Heyrovský Square 2, 162 06, Prague 6, CSFR

Authors and Affiliations

  1. Center for Management, Utilization and Protection of Water Resources and Department of Chemistry, Tennessee Technological University, 38501, Cookeville, TN, USA

    M. J. M. Wells

  2. Department of Pharmacal Sciences, School of Pharmacy, Auburn University, 36849, AL, USA

    C. R. Clark

  3. Laboratory for Infectious Diseases, Charles University and State Drug Control Institute, Prague, CSFR

    V. Rehak & J. Sabartova

Authors
  1. M. J. M. Wells
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  2. C. R. Clark
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  3. V. Rehak
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  4. J. Sabartova
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Wells, M.J.M., Clark, C.R., Rehak, V. et al. Selectivity studies on branched and linear alkyl bonded stationary phases in reversed-phase liquid chromatography. Chromatographia 32, 365–372 (1991). https://doi.org/10.1007/BF02321435

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  • DOI: https://doi.org/10.1007/BF02321435

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