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Refractive index detection in microbore column liquid chromatography

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Summary

An existing commercial refractive index detector was modified for use with microbore column LC systems. The detector utilizes the Fresnel method. The effect of band dispersion and dilution at the detector side is of extreme importance in connection with the miniaturized LC system. In the modified model the original heat-exchanger tube was removed and a stainless steel capillary was used for heat-exchanging. Gaskets having different cell volumes were also examined with respect to band broadening and sensitivity. The detection limit was 10ng for di-n-pentyl phthalate. The examples include the detection of phthalates, alcohols, n-paraffins, and kerosine.

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References

  1. R. R. Ryall, H. D. Kessier, Jr., Amer. Lab.,14 (2), 49 (February 1982).

    Google Scholar 

  2. M. Novotny, Anal. Chem.,53, 1294A (1981).

    Google Scholar 

  3. P. Kucera, ed., Microcolumn High-Performance Liquid Chromatography, Journal of Chromatography Library (Vol. 28), Elsevier, Amsterdam, 1984.

    Google Scholar 

  4. M. V. Novotny, D. Ishii, eds. Microcolumn Separations —Columns, Instrumentation and Ancillary Techniques, Journal of Chromatography Library (Vol. 30), Elsevier, Amsterdam, 1985.

    Google Scholar 

  5. R. P. W. Scott, P. Kucera, J. Chromatogr.,169, 51 (1979).

    Google Scholar 

  6. P. J. Naish, D. P. Goulden, C. V. Perkins, Chromatographia,20, 335 (1985).

    Google Scholar 

  7. S. Folestad, L. Johnson, B. Josefsson, B. Galle, Anal. Chem.,54, 925 (1982).

    Google Scholar 

  8. E. J. Caliguri, P. Capella, L. Bottari, I. N. Mefford, Anal. Chem.,57, 2423 (1985).

    Google Scholar 

  9. H. Menet, P. Gareil, M. Caude, R. Rosset, Chromatographia,18, 73 (1984).

    Google Scholar 

  10. G. Taylor, Proc. Roy. Soc. (London),219A, 196 (1953).

    Google Scholar 

  11. J. H. Knox, ed., High-Performance Liquid Chromatography, Edinburgh University Press, Edinburgh, 1978, p. 118.

    Google Scholar 

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Authors and Affiliations

  1. School of Materials Science, Toyohashi University of Technology, 440, Toyohashi, Japan

    C. Fujimoto, T. Morita & K. Jinno

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  1. C. Fujimoto
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  2. T. Morita
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  3. K. Jinno
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Fujimoto, C., Morita, T. & Jinno, K. Refractive index detection in microbore column liquid chromatography. Chromatographia 22, 91–94 (1986). https://doi.org/10.1007/BF02257306

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  • DOI: https://doi.org/10.1007/BF02257306

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