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Quantitative in situ thin-layer chromatography of ergocalciferol in multivitamin tablets

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Summary

A TLC method is reported for the determination of ergocalciferol in multivitamin tablets. Silica Gel HF254 is used as sorbent and cyclohexane-ethyl acetate as mobile phase. The concentration of the separated vitamin is determined by direct UV absorption scanning of the vitamin spot on the chromatogram. Results are reliable with samples containing between 0.05 and 3.0 μg of ergocalciferol. For comparison purposes ergocalciferol determination was carried out after TLC separation with the spectro-colorimetric antimony trichloride-acetylchloride method.

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Authors and Affiliations

  1. Research Institute of KRKA Pharmaceutical Works, Novo Mesto, Jugoslavia

    M. Širec & Lj. Mikša

  2. Institute for Drug Control, Zagreb, Jugoslavia

    T. Bičan-Fišter

  3. Research Institute of LEK Pharmaceutical and Chemical Works, Ljubljana, Jugoslavia

    M. Prošek & E. Kučan

Authors
  1. M. Širec
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  2. Lj. Mikša
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  3. T. Bičan-Fišter
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  4. M. Prošek
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  5. E. Kučan
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Širec, M., Mikša, L., Bičan-Fišter, T. et al. Quantitative in situ thin-layer chromatography of ergocalciferol in multivitamin tablets. Chromatographia 11, 217–219 (1978). https://doi.org/10.1007/BF02302396

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  • DOI: https://doi.org/10.1007/BF02302396

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