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Intercisternal substances of the Golgi apparatus

Unstacking of plant dictyosomes using chaotropic agents

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Summary

In media of high ionic strength, neutral pH, low temperature, and varying ion composition, plant dictyosomes were disassembled into component cisternae. The effective ions included phosphotungstate and several halides. Constituents of the intercisternal or bonding regions were revealed through electron microscope analysis. These included intercisternal elements and electrontransparent plaques of undetermined composition. The intercisternal plaques were confined to the central platelike regions of cisternae and were distinct from the intercisternal fibers. The findings demonstrate that plant dictyosomes can be dissociated into component cisternae. With monovalent halide salts, the unstacking process was sufficiently mild to reveal constituents of the intercisternal region as well as yield intact single cisternae.

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Mollenhauer, H.H., Morré, D.J. & Totten, C. Intercisternal substances of the Golgi apparatus. Protoplasma 78, 443–459 (1973). https://doi.org/10.1007/BF01275779

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