Summary
In media of high ionic strength, neutral pH, low temperature, and varying ion composition, plant dictyosomes were disassembled into component cisternae. The effective ions included phosphotungstate and several halides. Constituents of the intercisternal or bonding regions were revealed through electron microscope analysis. These included intercisternal elements and electrontransparent plaques of undetermined composition. The intercisternal plaques were confined to the central platelike regions of cisternae and were distinct from the intercisternal fibers. The findings demonstrate that plant dictyosomes can be dissociated into component cisternae. With monovalent halide salts, the unstacking process was sufficiently mild to reveal constituents of the intercisternal region as well as yield intact single cisternae.
Similar content being viewed by others
References
Amos, W. B., andA. V. Grimstone, 1968: Intercisternal material in the Golgi body ofTrichomonas. J. Cell Biol.38, 466–471.
Bradley, D. E., 1965: The structure of the head, collar and base-plate of “T-even” type bacteriophages. J. Gen. Microbiol.38, 395–408.
Bryan, J., 1970: On the reconstitution of the crystalline components of the sea urchin fertilization membrane. J. Cell Biol.45, 606–614.
Catterall, W. A., andP. L. Pedersen, 1970: Effects of phosphotungstic acid and silicotungstic acid on respiration and integrity of rat liver mitochondria. Biochem. Biophys. Res. Commun.38, 400–405.
Cunningham, W. P., D. J. Morré, andH. H. Mollenhauer, 1966: Structure of isolated plant Golgi apparatus revealed by negative staining. J. Cell Biol.28, 169–179.
Franke, W. W., J. Kartenbeck, S. Krien, W. J. van der Woude, U. Scheer, andD. J. Morré, 1972: Inter- and intracisternal elements of the Golgi apparatus: A system of membrane-to-membrane cross links. Z. Zellforsch.132, 365–380.
Franke, W. W., D. J. Morré, B. Deumling, R. D. Cheetham, J. Kartenbeck, E.-D. Jarasch, andH.-W. Zentgraf, 1971: Synthesis and turnover of membrane proteins in rat liver: An examination of the membrane flow hypothesis. Z. Naturforsch.26 b, 1031–1039.
Ganguly, P., 1966: The negative staining of protein molecules. Biochim. Biophys. Acta112, 565–572.
—, andD. N. Misra, 1965: II. Interaction of phosphotungstate with haemoglobin. J. molec. Biol.12, 385–393.
Hatefi, Y., andW. G. Hanstein, 1969: Solubilization of particulate proteins and non-electrolytes by chaotropic agents. Proc. nat. Acad. Sci. (USA)62, 1129–1136.
Horne, R. W., 1967: The effects of negative stains on lipids and proteins observed in the electron microscope. Protoplasma63, 212–213.
Junger, E., andH. Reinauer, 1969: Liquid crystalline phases of hydrated phosphatidylethanolamine. Biochim. biophys. Acta183, 304–308.
Mollenhauer, H., 1965: An intercisternal structure in the Golgi apparatus. J. Cell Biol.24, 504–511.
—, andD. J. Morré, 1966: Golgi apparatus and plant secretion. Ann. Rev. Plant Physiol.17, 27–46.
— —, 1972: Intercisternal substances of the Golgi apparatus, zones of exclusion, and other “invisible” structures that contribute to subcellular compartmentalization. What's New In Plant Physiol.4 (No. 5), 1–4.
- C.Totten, and K.Acuff, 1971: Separation of Golgi apparatus cisternae by solubilization of intercisternal bonding substances. Abst. 11th Annual Meeting of the American Soc. Cell Biol., New Orleans, Louisiana, 1971. p. 345.
Morré, D. J., 1971: Isolation of Golgi apparatus. Methods in Enzymol.22, 130–148.
—, andH. H. Mollenhauer, 1964: Isolation of the Golgi apparatus from plant cells. J. Cell Biol.23, 295–305.
- - and C. E.Bracker, 1971: Origin and continuity of Golgi apparatus. In: Results and problems in cell differentiation. Vol. II: Origin and continuity of cell organelles (Reinert andUrsprung, eds.), pp. 82–126.
— —, andJ. E. Chambers, 1965: Glutaraldehyde stabilization as an aid to Golgi apparatus isolation. Exp. Cell Res.38, 672–675.
Papahadjopoulos, D., andN. Miller, 1967: Phospholipid model membranes. I. Structural characteristics of hydrated liquid crystals. Biochim. Biophys. Acta135, 624–638.
Pease, D. C., 1970: Phosphotungstic acid as a specific electron stain for complex carbohydrates. J. Histochem. Cytochem.18, 455–458.
Sanyal, A. B., P. Ganguly, andN. N. Das Gupta, 1964: Interaction of phosphotungstate with haemoglobin. J. molec. Biol.8, 325–332.
Turner, F. R., andW. G. Whaley, 1965: Intercisternal elements of the Golgi apparatus. Science147, 1303–1304.
Wolfgang, J. M., M. A. Smith, andB. F. Trump, 1972: Sructural and functional effects of the negative stains silicotungstic acid, phosphotungstic acid, and ammonium molybdate on rat kidney mitochondria. Lab. Invest.27, 372.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mollenhauer, H.H., Morré, D.J. & Totten, C. Intercisternal substances of the Golgi apparatus. Protoplasma 78, 443–459 (1973). https://doi.org/10.1007/BF01275779
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01275779