Summary
We have confirmed that the LLA-15 polypeptide ofLilium longiflorum is (a) tapetum specific with some expression possible in the adjacent middle layer cells and (b) relatively abundant as evidenced by the high density of gold particles localized to the tapetal cells. We have established that the protein is cytoplasmic and not associated with organelles, membranes, extracellular matrix or wall. We also report an amino acid composition of the molecule and a partial sequence which bears no resemblance to any protein yet described.
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Abbreviations
- BSA:
-
bovine serum albumin
- PMSF:
-
phenyl methyl sulfonyl fluoride
References
Aguirre PJ, Smith AG (1993) Molecular characterization of a gene encoding a cysteine-rich protein preferentially expressed in anthers ofLycopersicon esculentum. Plant Mol Biol 23: 477–487
Altschul SF, Gish W, Miller W, Mayers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403–410
Bucciaglia PA, Smith AG (1994) Cloning and characterization ofTag 1, a tobacco anther β-1,3-glucanase expressed during tetrad dissolution. Plant Mol Biol 24: 903–914
Burnette WN (1981) Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112: 195–203
Capaldi RA, Vanderkooi G (1972) The low polarity of many membrane proteins. Proc Nat Acad Sci USA 69: 930–932
Chen R, Aguirre PJ, Smith AG (1994) Characterization of an anther- and tapetum-specific gene encoding a glycine-rich protein from tomato. J Plant Physiol 143: 651–658
Davis GL (1966) Systematic embryology of the angiosperms. Wiley, New York
De Block M, Debrouwer D (1993) Engineered fertility control in transgenicBrassica napus L.: histochemical analysis of anther development. Planta 189: 218–225
Delvallee I, Dumas C (1988) Anther development inZea mays: changes in protein, peroxidase, and esterase patterns. J Plant Physiol 132: 210–217
Dickinson HG, Sheldon JM (1990) The cell biological basis of exine formation inLilium sp. In: Proceedings of the VII Palynology Symposium APLE. University of Granada, CSIC, Granada, pp 17–29
Erickson RO (1948) Cytological and growth correlations in the flower bud and anther ofLilium longiflorum. Amer J Bot 35: 729–739
Faye L, Greenwood JS, Herman EM, Sturm A, Crispeels MJ (1988) Transport and posttranslational processing of the vacuolar enzyme a-mannosidase in jack-bean cotyledons. Planta 174: 271–282
Gould KS, Lord EM (1988) Growth of anthers inLilium longiflorum. A kinematic analysis. Planta 173: 161–171
Herich R, Lux A (1984) Electron microscopic study of tapetal cell nuclei degradation ofLilium henryi. Physiol Plant 20: 1–5
Herman EM (1988) Immunocytochemical localization of macromolecules with the electron microscope. Annu Rev Plant Physiol 39: 139–155
Heslop-Harrison J (1968) Tapetal origin of pollen coat substances inLilium. New Phytol 67: 779–786
Hird DL, Worrall D, Hodge R, Smartt S, Paul W, Scott R (1993) The anther-specific protein encoded by theBrassica napus andArabidopsis thaliana A6 gene displays similarity to β-1,3-glucanases. Plant J 4: 1023–1033
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Johri BM (ed) (1984) Embryology of angiosperms. Springer, Berlin Heidelberg New York Tokyo
Matsudaira P (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262: 10035–10038
Pacini E (1990) Tapetum and microspore function. In: Blackmore S, Knox RB (eds) Microspores: evolution and ontogeny. Academic Press, New York, pp 213–237
—, Franchi GG (1991) Diversification and evolution of the tapetum. In: Blackmore S, Barnes SH (eds) Pollen and spores — patterns of diversification. Clarendon, Oxford, pp 301–316
— — (1993) Role of the tapetum in pollen and spore dispersal. Plant Syst Evol [Suppl] 7: 1–11
Paul W, Hodge R, Smartt S, Draper J, Scott R (1992) The isolation and characterisation of the tapetum-specificArabidopsis thaliana A9 gene. Plant Mol Biol 19: 611–622
Pfeiffer NE (1935) Development of the floral axis and new bud in imported Easter lilies. Contrib Boyce Thompson Inst 7: 311–321
Reznickova SA, Willemse MTM (1980) Formation of pollen in the anther ofLilium. II. The function of surrounding tissues in the formation of pollen and pollen wall. Acta Bot Neerl 29: 141–156
Roberts MR, Foster GD, Blundell RP, Robinson SW, Kumar A, Draper J, Scott R (1993) Gametophytic and sporophytic expression of an anther-specificArabidopsis thaliana gene. Plant J 3: 111–120
Schägger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166: 368–379
Smith AG, Gasser CS, Budelier KA, Fraley RT (1990) Identification and characterization of stamen- and tapetum-specific genes from tomato. Mol Gen Genet 222: 9–16
Vergne P, Dumas C (1988) Isolation of viable wheat male gametophytes of different stages of development and variations in their protein patterns. Plant Physiol 88: 969–972
Wang CS, Walling LL, Eckard KJ, Lord EM (1992a) Patterns of protein accumulation in developing anthers ofLilium longiflorum correlate with histological events. Amer J Bot 79: 118–127
— — — — (1992b) Immunological characterization of a tapetal protein in developing anthers ofLilium longiflorum. Plant Physiol 99: 822–829
— —, Gu YQ, Ware CF, Lord EM (1994) Two classes of proteins and mRNAs inLilium longiflorum L. identified by human vitronectin probes. Plant Physiol 104: 711–717
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Balsamo, R.A., Wang, J.L., Eckard, K.J. et al. Immunogold localization of a developmentally regulated, tapetal-specific, 15 kDa lily anther protein. Protoplasma 189, 17–25 (1995). https://doi.org/10.1007/BF01280288
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DOI: https://doi.org/10.1007/BF01280288