Summary
A heterogeneous distribution of H+-ATPase was visualized in germinated pollen ofLilium longiflorum using monoclonal antibodies raised against plasma membrane H+-ATPase. Immunolocalization studies of protoplasts and subprotoplasts derived from pollen tubes and sectioned pollen grains and pollen tubes show that H+-ATPases are abundant in the plasma membrane of pollen grains but are absent or sparsely distributed in the plasma membrane of pollen tubes. This polar distribution of H+-ATPases is probably the basis of the endogenous current pattern measured in growing lily pollen and involved in pollen tube tip growth.
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Abbreviations
- BSA:
-
bovine serum albumine
- Hepes:
-
N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid
- Mes:
-
2-(N-morpholino)-ethane sulphonic acid
- PBS:
-
phosphate buffered saline
- Pipes:
-
piperazine-N,N′-bis(2-ethanesulfonic acid)
- Tris:
-
2-amino-2-hydroxymethyl-1,3-propandiol
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Obermeyer, G., Lützelschwab, M., Heumann, H.G. et al. Immunolocalization of H+-ATPases in the plasma membrane of pollen grains and pollen tubes ofLilium longiflorum . Protoplasma 171, 55–63 (1992). https://doi.org/10.1007/BF01379280
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DOI: https://doi.org/10.1007/BF01379280