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Cytoplasmic free calcium in single cells of centric diatoms. The use of fura-2

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Summary

Cytoplasmic free calcium concentration has been measured in centric diatoms using Fura-2. Cells hydrolysed Fura-2 acetoxymethyl ester and accumulated free Fura-2 in the cytoplasm. The [Ca2+] distribution in two species (Coccinodiscus concinnus andGuinardia flaccida) has been mapped using digital image analysis.

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Abbreviations

[Ca 2+cyt ]:

Cytoplasmic free calcium concentration

Quin-2/AM:

Quin-2 acetoxymethyl ester

Fura-2/AM:

Fura-2 acetoxymethyl ester DMSO dimethyl sulphoxide

CCTV:

closed circuit television

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Authors and Affiliations

  1. Institute for Marine Environmental Research, Plymouth

    D. Briton

  2. The Laboratory, Marine Biological Association of the United Kingdom, PL 1 2 PB, Citadel Hill, Plymouth, Devon, UK

    C. Brownlee & J. W. Wood

Authors
  1. C. Brownlee
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  2. J. W. Wood
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  3. D. Briton
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Brownlee, C., Wood, J.W. & Briton, D. Cytoplasmic free calcium in single cells of centric diatoms. The use of fura-2. Protoplasma 140, 118–122 (1987). https://doi.org/10.1007/BF01273720

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  • DOI: https://doi.org/10.1007/BF01273720

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