Abstract
Genetic variation in 126 clones of the planktonic diatom,Fragilaria capucina, collected from seven lakes from Manitoba to Texas, was determined by RAPD analysis. Five primers yielded 48 scorable RAPD fragments in 123 unique combinations. Patterns of genetic variation were analysed by cluster analysis, which showed that most clones grouped together according to the site from which they were collected. This pattern in genetic variation may be a result of the geographically disjunct nature of these populations and/or the different environmental conditions, especially temperature, in the different lakes.
Similar content being viewed by others
References
Atkinson, K. M., 1972: Birds as transporters of algae. — Brit. Phycol. J.7: 319–321.
Boileau, M. G., Hebert, P. D. N., Schwartz, S. S., 1992: Nonequilibrium gene frequency divergence: persistent founder effects in natural populations. — J. Evol. Biol.4: 25–39.
Brand, L. E., Murphy, L. S., Guillard, R. R. L., Lee, H.-T., 1981: Genetic variability and differentiation in the temperature niche component of the diatomThalassiosira pseudonana. — Mar. Biol.62: 103–110.
Dice, J. R., 1945: Measures of the amount of ecological association between species. — Ecology26: 297–302.
Drebes, G., 1977: Sexuality. — InWerner, D., (Ed.): The biology of diatoms, pp. 250–281. — Berkeley: University of California Press.
Felsenstein, J., 1989: PHYLIP: Phylogeny Inference Package, ver. 3.4. — Cladistics5: 164–166.
Gallagher, J. C., 1980: Population genetics ofSkeletonema costatum (Bacillariophyceae) in Narragansett Bay. — J. Phycol.16: 464–474.
, 1982: Physiological variation and electrophoretic banding patterns of genetically different seasonal populations ofSkeletonema costatum (Bacillariophyceae). — J. Phycol.18: 148–162.
Graham, J., McNicol, R. J., 1995: An examination of the ability of RAPD markers to determine the relationships within and betweenRubus species. — Theor. Appl. Genet.90: 1128–1132.
Guillard, R. R. L., 1975: Culture of phytoplankton for feeding marine invertebrates. — InSmith, W. L., Chanley, M. H., (Eds): Culture of marine invertebrate animals, pp. 29–60. — New York: Plenum.
, 1977: The ecology of marine planktonic diatoms. — InWerner, D, (Ed.): The biology of diatoms, pp. 372–469. — Berkeley: University of California Press.
, 1962: Studies of marine planktonic diatoms. I.Cyclotella nana Hustedt, andDetonula confervacea (Cleve)Gran. — Canad. J. Microbiol.8: 229–239.
Hasle, G. R., 1978: Some freshwater and brackish water species of the diatom genusThalassiosira Cleve. — Phycologia17: 263–292.
, 1983: The marine, planktonic diatomsThalassiosira oceanica sp. nov. andT. partheneia. — J. Phycol.19: 220–229.
Hebert, P. D. N., Wilson, C. C., 1994: Provincialism in plankton: endemism and allopatric speciation in AustralianDaphnia. — Evolution48: 1333–1349.
Hoshaw, R. W., Rosowski, J. R., 1973: Methods for microscopic algae. — InStein, J. R., (Ed.): Handbook of phycological methods.1: Culture methods and growth measurements, pp. 53–67. — Cambridge: Cambridge University Press.
Hutchinson, G. E., 1967: A treatise on limnology.2. Introduction to lake biology and the limnoplankton. — New York: Wiley.
Jaworski, G., Lund, J. W. G., 1970: Drought resistance and dispersal ofAsterionella formosa Hass. — Beih. Nova Hedwigia31: 37–48.
Krawiec, R. W., 1982: Autecology and clonal variability of the marine centric diatomThalassiosira rotula (Bacillariophyceae) in response to light, temperature and salinity. — Mar. Biol.69: 79–89.
Maniatis, T., Fritch, E. F., Sambrook, J., 1982: Molecular cloning: a laboratory manual. — Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
Mann, D. G., 1984: Observations on copulation ofNavicula pupula andAmphora ovalis in relation to the nature of diatom species. — Ann. Bot.54: 429–438.
, 1989: The species concept in diatoms: evidence for morphologically distinct, sympatric gamodemes in four epipelic species. — Pl. Syst. Evol.164: 215–237.
Megnegneau, B., Debets, F., Hoekstra, R. F., 1993: Genetic variability and relatedness in the complex group of blackAspergilli based on random amplification of polymorphic DNA. — Curr. Genet.23: 323–329.
Murphy, L. S., Guillard, R. R. L., 1976: Biochemical taxonomy of marine phytoplankton by electrophoresis of enzymes. I. The centric diatomsThalassiosira pseudonana andT. fluviatilis. — J. Phycol.12: 9–13.
Patrick, R., Reimer, C. W., 1966: The diatoms of the United States 1. — Acad. Nat. Sci. Philadelphia Monogr.13: 1–688.
Pipe, N. D., Buck, K. W., Brasier, C. M., 1995: Molecular relationships betweenOphiostoma ulmi and the NAN and EAN races ofO. novo-ulmi determined by RAPD markers. — Mycol. Res.99: 653–658.
Price, N. M., Thompson, P. A., Harrison, P. J., 1987: Selenium: an essential element for growth of the coastal marine diatomThalassiosira pseudonana. — J. Phycol.23: 1–9.
Round, F. E., 1981: The ecology of algae. — Cambridge: Cambridge University Press.
, 1990: The diatoms: biology and morphology of the genera. — Cambridge: Cambridge University Press.
Saitou, N., Nei, M., 1987: The neighbor-joining method: a new method for reconstructing phylogenetic trees. — Molec. Biol. Evol.4: 406–425.
Schindler, D. W., Beaty, K. G., Fee, E. J., Cruikshank, D. R., Debruyn, E. R., Findlay, D. L., Linsey, G. A., Shearer, J. A., Stainton, M. P., Turner, M. A., 1990: Effects of climatic warming on lakes of the Central Boreal Forest. — Science250: 967–970.
Sneath, P. H., Sokal, R. R., 1973: Numerical taxonomy. — San Francisco: Freeman.
Soudek, D. Jr.,Robinson, G. G. C., 1983: Electrophoretic analysis of the species and population structure of the diatomAsterionella. — Canad. J. Bot.61: 418–433.
Stabile, J. E., Gallagher, J. C., Wurtzel, E. T., 1990: Molecular analysis of intraspecific variation in the marine diatomSkeletonema costatum. — Biochem. Syst. Ecol.18: 5–9.
, 1992: Comparison of chloroplast DNA and allozyme variation in winter strains of the marine diatomSkeletonema costatum (Bacillariophyceae). — J. Phycol.28: 90–94.
Underhill, P. A., 1977: Nitrate uptake kinetics and clonal variability in the neritic diatomBiddulphia aurita. — J. Phycol.13: 170–176.
van Oppen, M. J. H., Diekmann, O. E., Wiencke, C., Stam, W. T., Olsen, J. L., 1994: Tracking dispersal routes: phylogeography of the Arctic-Antarctic disjunct seaweedAcrosiphonia arcta (Chlorophyta). — J. Phycol.30: 67–80.
, 1995: Genetic variation within and among North Atlantic and Baltic populations of the benthic algaPhycodrys rubens (Rhodophyta). — Eur. J. Phycol.30: 251–260.
Varadarajan, G. S., Prakash, C. S., 1991: A rapid efficient method for the extraction of total DNA from the sweet potato and its related species. — Pl. Mol. Biol. Reporter9: 6–12.
Vicario, F., Vendramin, G. G., Rossi, P., Liò, P., Giannini, R., 1995: Allozyme, chloroplast DNA and RAPD markers for determining genetic relationships betweenAbies alba and the relic population ofAbies nebrodensis. — Theor. Appl. Genet.90: 1012–1018.
Weider, L. J., Beaton, M. J., Hebert, P. D. N., 1987: Clonal diversity in high arctic populations ofDaphnia pulex: a polyploid apomictic complex. — Evolution41: 1335–1346.
Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., Tingey, S. V., 1990: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. — Nucl. Acids Res.18: 6531–6535.
Zechman, F. W., Zimmer, E. A., Theriot, E. C., 1994: Use of ribosomal DNA internal transcribed spacers for phylogenetic studies in diatoms. — J. Phycol.30: 507–512.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lewis, R.J., Jensen, S.I., DeNicola, D.M. et al. Genetic variation in the diatomFragilaria capucina (Fragilariaceae) along a latitudinal gradient across North America. Pl Syst Evol 204, 99–108 (1997). https://doi.org/10.1007/BF00982534
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00982534