Skip to main content
Log in

Interspecific relationship of ten European orchid species as revealed by enzyme electrophoresis

  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Ten species of orchid plants belonging to the generaOrchis (7),Dactylorhiza (2), andGymnadenia (1) were analyzed by enzyme electrophoresis. Each species can be identified by a combination of enzyme bands different from those of all other species examined. The electrophoretic data were used for the construction of phenetic and phylogenetic trees with the help of computer programs. The trees were almost identical regardless which method was used. Our results differ considerably from a classification based on morphological evidence. The electrophoretic data indicate that the genusOrchis is not a monophyletic group.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ayala, F. J., 1975: Genetic differentiation during the speciation process. — InDobzhansky, T., Hecht, M., Steere, W. C. (Eds.): Evolutionary biology8, pp. 1–78. — New York: Plenum Press.

    Google Scholar 

  • —, 1976: Molecular evolution. — Sunderland, Massachusetts: Sinauer Ass.

    Google Scholar 

  • Baumann, H., Künkele, S., 1982: Die wildwachsenden Orchideen Europas. — Stuttgart: Kosmos.

    Google Scholar 

  • —, 1986: Die GattungOphrys. Eine taxonomische Übersicht. — Mitt. Bl. Arbeitskr. Heim. Orch. Baden-Württ.18: 305–688.

    Google Scholar 

  • Buth, D. G., 1984: The application of electrophoretic data in systematic studies. — Ann. Rev. Ecol. Syst.15: 501–522.

    Google Scholar 

  • Buttler, K. P., 1986: Orchideen. — München: Mosaik Verlag.

    Google Scholar 

  • Danesch, O., Danesch, E., Ehrendorfer, F., Ehrendorfer, K., 1973: Über die Hybridisierung einigerDactylorhiza-Sippen in Tirol. — Veröff. Mus. Ferd. Innsbruck53: 95–124.

    Google Scholar 

  • —, 1975: Hybriden und hybridogene Sippen ausOphrys bertoloni undO. atrata (Orchidaceae). — Pl. Syst. Evol.124: 7–123.

    Google Scholar 

  • Ehrendorfer, F., 1980: Hybridisierung, Polyploidie und Evolution bei europäischmediterranen Orchideen. — Die Orchidee, Sonderheft 1980: 15–34.

    Google Scholar 

  • Farris, J. S., 1970: Methods of computing Wagner trees. — Syst. Zool.19: 83–92.

    Google Scholar 

  • —, 1972: Estimating phylogenetic trees from distance matrices. — Amer. Nat.106: 645–668.

    Google Scholar 

  • —, 1981: Distance data in phylogenetic analyses. — InFunk, V. A., Brooks, D. R., (Eds.): Advances in cladistics: proceedings of the first meeting of the Willi Hennig Society. — New York Bot. Gard., Bronx, 3–23.

    Google Scholar 

  • —, 1985: Distance data revisited. — Cladistics1: 67–85.

    Google Scholar 

  • Felsenstein, J., 1982: Numerical methods for inferring evolutionary trees. — Quart. Rev. Biol.57: 379–404.

    Google Scholar 

  • Ferguson, A., 1980: Biochemical systematics and evolution. — Glasgow, London: Blackie.

    Google Scholar 

  • Fitch, W. M., Margoliash, E., 1967: Construction of phylogenetic trees. — Science155: 279–284.

    PubMed  Google Scholar 

  • Harris, H., Hopkinson, D. A., 1976: Handbook of enzyme electrophoresis in human genetics. — Amsterdam, New York: North Holland.

    Google Scholar 

  • Hedrik, P. W., 1983: Genetics of populations. — New York: Van Nostrand Reinhold Company.

    Google Scholar 

  • Hennig, W., 1982: Phylogenetische Systematik. — Berlin: Parey.

    Google Scholar 

  • Kluge, A. G., Farris, J., 1969: Quantitative phyletics and the evolution of anurans. — Syst. Zool.18: 1–32.

    Google Scholar 

  • Mickevich, M. F., 1982: Transformation series analysis. — Syst. Zool.31: 461–478.

    Google Scholar 

  • —, 1976: Congruence between morphological and allozyme data in evolutionary inference and character evolution. — Syst. Zool.25: 260–270.

    Google Scholar 

  • Mossakowski, D., 1987: Kontroverse Ansätze in der phylogenetischen Methodik. — Mitt. Deutsch. Ges. Allg. Angew. Ent.5: 94–103.

    Google Scholar 

  • Nei, M., 1972: Genetic distance between populations. — Amer. Nat.106: 283–292.

    Google Scholar 

  • Nelson, E., 1976: Monographie und Ikonographie der OrchideengattungDactylorhiza. — Zürich: Speich AG.

    Google Scholar 

  • Nomenclature Committee of the International Union of Biochemistry, 1984: Enzyme nomenclature. — New York, London: Academic Press.

    Google Scholar 

  • Patterson, C., 1987: Molecules and morphology in evolution: conflict or compromise? — Cambridge: Cambridge University Press.

    Google Scholar 

  • Pinsker, W., Buruga, J., 1982: Comparative study of allozyme variation in six species of theDrosophila obscura-group. — Z. Zool. Syst. Evolut. Forsch.20: 53–63.

    Google Scholar 

  • Schlegel, M., 1985: Comparative study of allozyme variation in eight species of hypotrichous ciliates (Polyphymenophora, Ciliophora). — Z. Zool. Syst. Evolut. Forsch.23: 171–183.

    Google Scholar 

  • Sneath, P. H. A., Sokal, R. R., 1973: Numerical taxonomy. — San Francisco: Freeman.

    Google Scholar 

  • Soó, R. v., 1968: Die Geschichte der Erforschung der GattungOrchis (sensu lato), besonders vonDactylorhiza. — Die Orchidee, Sonderheft 1968: 7–19.

    Google Scholar 

  • Steinbrück, G., Schlegel, M., Dahlström, I., Röttger, B., 1986: Characterization of interspecific hybrids betweenOrchis mascula andO. pallens (Orchidaceae) by enzyme electrophoresis. — Pl. Syst. Evol.153: 229–241.

    Google Scholar 

  • Sundermann, H., 1980a: Modelle zur Evolution innerhalb der GattungOphrys. — Die Orchidee, Sonderheft 1980: 44–54.

    Google Scholar 

  • —, 1980b: Europäische und mediterrane Orchideen. — Hildesheim: Brücke-Verlag, Kurt Schmersow.

    Google Scholar 

  • Vermeulen, P., 1947: Studies onDactylorchis. — Diss. Utrecht.

  • —, 1972: Übersicht zur Systematik und Taxonomie der GattungOrchis s. str. — Jahresber. Naturwiss. Ver. Wuppertal25: 22–36.

    Google Scholar 

  • —, 1980: Einige kritische Probleme in der Monographie der OrchideengattungDactylorhiza (DeNecker)Nevski vonE. Nelson. — Die Orchidee, Sonderheft 1980: 168–174.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schlegel, M., Steinbrück, G., Hahn, K. et al. Interspecific relationship of ten European orchid species as revealed by enzyme electrophoresis. Pl Syst Evol 163, 107–119 (1989). https://doi.org/10.1007/BF00936158

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00936158

Key words

Navigation