Abstract
During the course of mitochondrial DNA sequencing ofDictyostelium discoideum, a sequence with a tRNA-like structure and two genes for tRNAGin(UUG) and tRNATrp(CCA) were found downstream of the gene for large subunit rRNA. The existence of tRNATrp with CCA anticodon supports the finding that UGA codon is not a tryptophan codon inD. discoideum mitochondria. Interestingly, the tRNA gene-like sequence (t-element) has TTA at the anticodon position. Northern blot analysis showed that, in low molecular mass mitochondrial RNA fraction of growth-phase cells and developmental stage cells, a mature transcript of the ele ment could not be detected in the tRNA region on an ureadenatured 15% polyacrylamide gel, although there were several bands in the higher molecular mass region, indicating the actual transcription of the t-element. Southern blot analysis for total and mitochondrial DNA showed that the element exists as a single copy, only in the mitochondrial DNA but not in the nuclear DNA.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- LSU:
-
large subunit
- mt:
-
mitochondrial
References
Angata, K., Kuroe, K., Yanagisawa, K. andTanaka, Y. 1995a. Codon usage, genetic code and phylogeny ofDictyostelium discoideum mitochondrial DNA as deduced from a 7.3-kb region. Curr. Genet.27: 249–256.
Angata, K., Ogawa, S., Yanagisawa, K. andTanaka, Y. 1995b. A group-1 intron in the mitochondrial large-subunit ribosomal RNA-encoding gene ofDictyostelium discoideum: same site localization in alga and in vitro self-splicing. Gene153: 49–55.
Benner, S.A., Ellington, A.D. andTauer, A. 1989. Modern metabolism as a palimpsest of the RNA world. Proc. Natl. Acad. Sci. USA86: 7054–7058.
Cole, R.A. andWilliams, K.L. 1994. TheDictyostelium discoideum mitochondrial genome: a primodial system using the universal code and encoding hydrophilic proteins atypical of metazoan mitochondrial DNA. J. Mol. Evol.39: 579–588.
Crick, F.H.C. 1958. On protein synthesis. Symp. Soc. Exptl. Biol.12: 138–163.
Fernandez-Moreno, M.A., Caballero, J.L., Hopwood, D.A. andMalpartida, F. 1991. Theact cluster contains regulatory and antibiotic export genes, direct targets for translational control by thebldA tRNA gene of Streptomyces. Cell66: 769–780.
Franke, J. andKessin, R.H. 1977. A defined minimal medium for axenic strains ofDictyostelium discoideum. Proc. Natl. Acad. Sci. USA74: 2157–2161.
Fukuhara, H. 1982. Restriction map and gene organization of the mt DNA fromD. discoideum. Biol. Cell46: 321–324.
Gorovsky, M.A. 1970. Studies on nuclear structure and function inTetrahymena pyriformis. J. Cell Biol.47: 619–623.
Hanic-Joyce, P.J., Spencer, D.F. andGray, M.W. 1990.In vitro processing of transcripts containing novel tRNA-like sequences (‘t-elements’) encoded by wheat mitochondrial DNA. Plant Mol. Biol.15: 551–559.
Joyce, P.B.M., Spencer, D.F. andGray, M.W. 1988. Multiple sequence rearrangements accompanying the duplication of a tRNApro gene in wheat mitochondrial DNA. Plant Mol. Biol.11: 833–843.
Kikuchi, Y., Sasaki, N. andAndo-Yamagami, Y. 1990. Cleavage of tRNA within the mature tRNA sequence by the catalytic RNA of RNase P: Implication for the formation of the primer tRNA fragment for reverse transcription incopia retrovirus-like particles. Proc. Natl. Acad. Sci. USA87: 8105–8109.
Kimmel, A.R. 1988. Molecular Biology ofDictyostelium Development. Alan R. Liss, New York.
Komine, Y., Kitabatake, M., Yokogawa, T., Nishikawa, K. andInokuchi, H. 1995. A tRNA-like structure is present in 10Sa RNA, a small stable RNA fromEscherichia coli. Proc. Natl. Acad. Sci. USA91: 9223–9227.
Nashimoto, M. 1993. 3'truncated tRNAArg is essential forin vitro specific cleavage of partially synthesized mouse 18S rRNA. Nucleic Acids Res.21: 4696–4702.
Orii, H., Tanaka, Y. andYanagisawa, K. 1989. Sequence organization and gene expression of PDG1, a plasmid found in a wild isolate ofDictyostelium. Nucleic Acids Res.17: 1395–1408.
Osawa, S. 1995. Evolution of the Genetic Code. Oxford Univ. Press, Oxford.
Sambrook, J., Fritsch, E.F. andManiatis, T. 1989. Molecular Cloning: A Laboratory Manual. 2nd Ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Sanger, F., Nicklen, S. andCoulson, A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA74: 5463–5467.
Schon, A., Krupp, G., Gough, S., Berry-Lowe, S., Kannangara, C.G. andSoll, D. 1986. The RNA required in the first step of chlorophyll biosynthesis is a chloroplast glutamate tRNA. Nature322: 281–284.
Spencer, D.F., Schnare, M.N., Coulthart, M.B. andGray, M.W. 1992. Sequence and organization of a 7.2 kb region of wheat mitochondrial DNA containing the large subunit (26S) rRNA gene. Plant Mol. Biol.20: 354–352.
Sussman, M. 1966. Biochemical and genetic methods in the study of cellular slime mold development.In D. Prescott, ed., Methods in Cell Physiology, vol. 2, Academic Press, New York, pp. 397–410.
Takeuchi, I. 1960. The correlation of cellular changes with succinic dehydrogenase and cytochrome oxidase activities in the development of the cellular slime molds. Develop. Biol.2: 343–366.
Tamura, F., Nishimura, S. andOhki, M. 1984. TheE. coli divE mutation, which differentially inhibits synthesis of certain proteins, is in tRNASer. EMBO J.3: 1103–1107.
Tanaka, Y., Kuroe, K., Angata, K. andYanagisawa, K. 1990. ORF209 ofDictyostelium discoideum mitochondrial DNA has a homologue in chloroplast DNA. Plant Mol. Biol.15: 659–660.
Tasaka, M. andMaeda, Y. 1983. Ultrastructural changes of the two types of differentiated cells during the migration and early culmination stages ofDictyostelium discoideum. Develop. Growth Different.25: 353–360.
Yokogawa, T., Kumazawa, Y., Miura, K. andWatanabe, K. 1989. Purification and characterization of two serine isoacceptor tRNAs from bovine mitochondria by using a hybridization assay method. Nucleic Acids Res.17: 2623–2638.
Yu, W. andSpreitzer, R.J. 1991. Sequences oftrnR-ACG andpetD that contain a tRNA-like element within the chloroplast genome ofChlamydomonas reinhardtii. Nucleic Acids Res.19: 957.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pi, M., Angata, K., Ikemura, T. et al. Analysis of a tRNA gene-like sequence (t-element) with TTA at the anticodon position in the mitochondrial DNA ofDictyostelium discoideum . J. Plant Res. 109, 1–6 (1996). https://doi.org/10.1007/BF02344281
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02344281