Abstract
The role of β-galactosidase repression in glucose inhibition of lactose utilization was studied inEscherichia coli. Escherichia coli 3300 constitutively produces β-galactosidase even in the presence of glucose. When this strain was grown in a mixture of glucose and lactose, lactose utilization did not occur until glucose was depleted. The addition of glucose to a 3300 culture grown in lactose immediately caused a permanent inhibition of lactose utilization and only a mild transient repression of β-galactosidase. Exogenous cyclic adenosine monophosphate (AMP) did not overcome the glucose inhibition of lactose utilization but did relieve the transient repression. Thus glucose inhibition of lactose utilization is not related to β-galactosidase repression and is independent of cyclic AMP.
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Literature Cited
Bollum, F. J. 1968. Filter paper disk techniques for assaying radioactive macromolecules, pp. 169–173. In: Grossman, L., Moldave, K. (eds.), Methods in enzymology, vol. 12 part B. New York, London: Academic Press.
Epstein, W., Naono, S., Gros, F. 1966. Synthesis of enzymes of the lactose operon during diauxic growth ofEscherichia coli. Biochemical and Biophysical Research Communications24:588–592.
Khan, S. R., Yamazaki, H. 1974. Inapparent correlation between guanosine tetraphosphate levels and RNA contents inEscherichia coli. Biochemical and Biophysical Research Communications59:125–132.
Magasanik, B. 1970. Glucose effects: Inducer exclusion and repression, pp. 189–219. In: Beckwith, J. R., Zipser, D. (eds.), The lactose operon. New York: Cold Spring Harbor Laboratory.
Miller, J. H. 1972. Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory.
Naono, S., Rouvière, J., Gros, F. 1965. Preferential transcription of the lactose operon during the diauxic growth ofEscherichia coli. Biochemical and Biophysical Research Communications,18:664–674.
Okada, T., Ueyama, K., Niiya, S., Kanazawa, H., Futai, M., Tsuchiya, T. 1981. Role of inducer exclusion in preferential utilization of glucose over melibiose in diauxic growth ofEscherichia coli. Journal of Bacteriology146:1030–1037.
Pardee, A. B., Jacob, F., Monod, J. 1959. The genetic control and cytoplasmic expression of “inducibility” in the synthesis of β-galactosidase byE. coli. Journal of Molecular Biology1:165–178.
Pastan, I., Perlman, R. 1970. Cyclic adenosine monophosphate in bacteria. Science169:339–344.
Tyler, B., Loomis, W. F., Jr., Magasanik, B. 1967. Transient repression of thelac operon. Journal of Bacteriology94:2001–2011.
Ullmann, A., Danchin, A. 1982. Role of cyclic AMP in bacteria, in press. In: Greengard, P., Robison, G. A. (eds.), Advances in cyclic nucleotide research, vol. 15. New York: Raven Press.
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Fraser, A.D.E., Yamazaki, H. Significance of β-galactosidase repression in glucose inhibition of lactose utilization inEscherichia coli . Current Microbiology 7, 241–244 (1982). https://doi.org/10.1007/BF01568806
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DOI: https://doi.org/10.1007/BF01568806