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Some group N plasmids makeEscherichia coli K-12 strain AB1157 dependent on exogenous purine

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Abstract

Escherichia coli K-12 strain AB1157 given the conjugative group N plasmid R46 (or its derivative, pKM101, or plasmid R384N) grew only very slowly on defined medium containing the known growth-factor requirements of AB1157, which do not include any purine. Addition of adenine or hypoxanthine (or their nucleosides) restored normal growth; guanine and xanthine (and their nucleosides) were ineffective, because of thegpt defect caused by deletionproA2. Variants of AB1157(R46) able to grow rapidly on defined medium without purine were tetracycline-sensitive and/or transfer-defective; an, R46 gene,slo, causing purine auxotrophy, is inferred to be betweentet andtra.

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Authors and Affiliations

  1. Department of Microbiology, Shiraz University, Shiraz, Iran

    Nahid S. Waleh

  2. Department of Medical Microbiology, Stanford University School of Medicine, 94305, Stanford, California, USA

    Bruce A. D. Stocker

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  1. Nahid S. Waleh
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  2. Bruce A. D. Stocker
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Waleh, N.S., Stocker, B.A.D. Some group N plasmids makeEscherichia coli K-12 strain AB1157 dependent on exogenous purine. Current Microbiology 6, 337–341 (1981). https://doi.org/10.1007/BF01567008

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