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Preparation of R-type lipopolysaccharides ofAcinetobacter calcoaceticus by EDTA-salt extraction

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Abstract

An EDTA-salt extraction method is presented for the preparation of R-type LPS fromAcinetobacter calcoaceticus. Since the cell extract is free of nucleic acids and contains only few protein contaminations, it is easily purified by Sephadex G-200 chromatography and CaCl2 precipitation. Yield and chemical composition are similar to the LPS prepared by the phenol-chloroform-light petroleum method of Brade and Galanos [Eur J Biochem 122:233–237]. Significant differences were found in the fatty acid composition between both preparations, mainly with respect to the proportions of unsaturated fatty acids and chain length. This finding may be attributed to heterogeneities demonstrated within both R-type LPS isolated.

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Authors and Affiliations

  1. Department of Biochemistry, Section of Biological Sciences, Karl Marx University, Talstr. 33, 7010, Leipzig, German Democratic Republic

    Petra Borneleit, Bernd Blechschmidt, Peter Günther & Hans-Peter Kleber

  2. Central Institute of Molecular Biology, Academy of Sciences of the GDR, German Democratic Republic

    Rosel Blasig & Peter Franke

Authors
  1. Petra Borneleit
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  2. Bernd Blechschmidt
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  3. Rosel Blasig
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  4. Peter Franke
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  5. Peter Günther
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  6. Hans-Peter Kleber
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Borneleit, P., Blechschmidt, B., Blasig, R. et al. Preparation of R-type lipopolysaccharides ofAcinetobacter calcoaceticus by EDTA-salt extraction. Current Microbiology 19, 77–81 (1989). https://doi.org/10.1007/BF01570572

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