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Calmodulin defects cause the loss of Ca2+-dependent K+ currents in two pantophobiac mutants ofParamecium tetraurelia

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Summary

Two behavioral mutants ofParamecium tetraurelia, pantophobiacs A1 and A2, have single amino acid defects in the structure of calmodulin. The mutants exhibit several major ion current defects under voltage clamp: (i) the Ca2+-dependent K+ current activated upon depolarization ofParamecium is greatly reduced or missing in both mutants, (ii) both mutants lack a Ca2+-dependent K+ current activated upon hyperpolarization, and (iii) the Ca2+-dependent Na+ current is significantly smaller in pantophobiac A1 compared with the wild type, whereas this current is slightly increased in pantophobiac A2.

Other, minor defects include a reduction in peak amplitude of the depolarization-activated Ca2+ current in pantophobiac A2, increased rates of voltage-dependent inactivation of this Ca2+ current in both pantophobiac A1 and pantophobiac A2, and an increase in the time required for the hyperpolarization-activated Ca2+ current to recover from inactivation in the pantophobiacs.

The diversity of the pantophobiac mutations' effects on ion current function may indicate specific associations of calmodulin with a variety of Ca2+-related ion channel species inParamecium.

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Author notes

  1. Margaret A. Wallen-Friedman

    Present address: Yale University School of Medicine, 367 Cedar Street, 06510, New Haven, Connecticut

Authors and Affiliations

  1. Laboratory of Molecular Biology, University of Wisconsin-Madison, 53706, Madison, Wisconsin

    Robin R. Preston, Margaret A. Wallen-Friedman, Yoshiro Saimi & Ching Kung

  2. Neuroscience Training Program, University of Wisconsin-Madison, 53706, Madison, Wisconsin

    Margaret A. Wallen-Friedman

  3. Department of Genetics, University of Wisconsin-Madison, 53706, Madison, Wisconsin

    Ching Kung

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  1. Robin R. Preston
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  2. Margaret A. Wallen-Friedman
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  3. Yoshiro Saimi
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  4. Ching Kung
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Preston, R.R., Wallen-Friedman, M.A., Saimi, Y. et al. Calmodulin defects cause the loss of Ca2+-dependent K+ currents in two pantophobiac mutants ofParamecium tetraurelia . J. Membrain Biol. 115, 51–60 (1990). https://doi.org/10.1007/BF01869105

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