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A structural basis for the transphosphorylation of nucleotides with hydroxyapatite

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Abstract

The basis for transphosphorylation between nucleotides and hydroxyapatite (HA) has been explored. Using a recently-proposed model for the surface of HA and the structure of 2- and 3-membered polyphosphates, a reasonable atomic explanation can be shown for this reaction. Transphosphorylation has been found to result in a pyrophosphate on HA which is distinctive from pyrophosphate absorbed onto HA from solution. The models suggest that this distinction is due to a different orientation of the pyrophosphate on the surface of the HA depending on the origin of the pyrophosphate.

Résumé

La base de la transphosphorilation entre les nucléotides et l'hydroxyapatite (HA) est explorée. Utilisant un recent modèle de la surface de HA et de la structure de polyphosphates de 2 et 3 parties, une raisonnable explication atomique peut être montrée pour cette réaction. La transphosphorilation a produit un pyrophosphate sur HA qui est différent du pyrophosphate absorbé sur HA de la solution. Les modèles suggèrent que la distinction est dûe à une orientation différente du pyrophosphate sur la surface de HA dépendant de l'origine du pyrophosphate.

Zusammenfassung

Es wurde die Grundlage für die Transphosphorylierung zwischen Nukleotiden und Hydroxy-Apatit (HA) untersucht. Eine plausible atomare Darstellung dieser Reaktion ist möglich, wenn man kürzlich vorgeschlagene Modelle der Oberfläche von HA und der Struktur von zwei- und dreigliedrigen Polyphosphaten benutzt.

Man findet dann, daß Transphosphorylierung zu einem Pyrophosphat des HA führt, welches von dem Pyrophosphat, welches HA aus der Lösung absorbiert, unterschieden werden kann. Auf Grund der Modelle kann man annehmen, daß dieser Unterschied auf einer unterschiedlichen Orientierung des Pyrophosphats auf der Oberfläche des HA beruht, welche wiederum von der Herkunft des Pyrophosphats abhängt.

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Authors and Affiliations

  1. Department of Radiation Biology and Biophysics, University of Rochester School of Medicine and Dentistry, 14620, Rochester, New York

    D. R. Taves & R. C. Reedy

Authors
  1. D. R. Taves
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  2. R. C. Reedy
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Additional information

This paper is based partially on work performed under contract with U.S. Atomic Energy Commission at the University of Rochester Atomic Energy Project and also on a USPHS Dental Grant STD1 DE00175 and has been assigned Report No. UR-49-967. We wish to thankRobert L. Collin for suggestions in the preparation of this manuscript.

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Taves, D.R., Reedy, R.C. A structural basis for the transphosphorylation of nucleotides with hydroxyapatite. Calc. Tis Res. 3, 284–292 (1969). https://doi.org/10.1007/BF02058670

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  • DOI: https://doi.org/10.1007/BF02058670

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