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A hormone dependent calcium-binding protein in the mantle edge of the freshwater snailLymnaea stagnalis

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Summary

By means of gel filtration and ionexchange chromatography on calcium-saturated Chelex-100, a calcium-binding fraction was isolated from the mantle edge of the freshwater snailLymnaea stagnalis. This fraction was not present in other tissues. Treatment with trypsin caused a disappearance of the calcium-binding capacity, proving that the active substance in this fraction is a protein (calcium-binding protein; CaBP). Removal of the growth hormone-producing neuroendocrine light green cells resulted in a strong decrease of the amount of CaBP. It is concluded thatL. stagnalis possesses a hormone-dependent CaBP, probably responsible for the maintenance of a high calcium concentration in that part of the mantle that produces the outer crystalline layer of the shell.

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Authors and Affiliations

  1. Department of Biology, Free University, P.O. Box 7161, 1007 MC, Amsterdam, The Netherlands

    Arend A. Dogterom

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  1. Arend A. Dogterom
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  2. Albert Doderer
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Dogterom, A.A., Doderer, A. A hormone dependent calcium-binding protein in the mantle edge of the freshwater snailLymnaea stagnalis . Calcif Tissue Int 33, 505–508 (1981). https://doi.org/10.1007/BF02409481

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