Abstract
Altitude hypoxia does not induce any changes in the enzymatic systems related to oxygen consumption in guinea pigs native of the Peruvian high altitudes. The biochemical changes frequently found in high altitude animals are the result of exposure to the low temperature of this environment rather than to hypoxia. In the present work, mice were chronically exposed to hypobaric hypoxia and maintained at equal temperature as the sea level control group, and measurements of enzymatic activities of the three major oxygen consuming systems of the liver were carried out, i.e., mitochondria, microsomes and peroxisomes. The results obtained have confirmed that hypoxia has no apparent influence on these enzymatic systems.
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AEBI, H. (1974): In Methods of Enzymatic Analysis. Vol. 2, Academic Press. New York, 674.
APPELMANS, F., WATTIAUX, R. and DE DUVE, C. (1955): The association of acid phosphatase with a special class of cytoplasmic granules in rat liver. Biochem. J., 59: 438–445.
BARBASHOVA, Z. I. (1967): Studies on the mechanisms of resistance to hypoxia. A Review. Int. J. Biometeor., 11: 243–254.
BURTON, R. R. and SMITH, A. H. (1967): Effect of polycythemia and chronic hypoxia on heart mass in the chicken. J. appl. Physiol., 22: 782–785.
BUSTAMANTE, E. (1972): Distribución y caracterización enzimática de la problación subcelular en hígado de cobayo. M.S. Thesis, Universidad Peruana Cayetano Heredia.
CAMBA, E., MONTESTRUQUE, S. and ALVAREZ, J. (1980): Hypoxia and cold: Influence on cellular oxygen consuming systems in guinea pig liver. Life Sci., 27: 943–952.
COHEN, G., DEMBIEC, D. and MARCUS, J. (1970): Measurements of catalase activity in tissue extract. Anal. Biochem., 34: 30–38.
CORREA, A. M. (1978): Un modelo de hipoxia intermiente: Estudio en ratones de algunos parámetros sanguíneos cerebrales. B.S. Thesis, Universidad Peruana Cayetano Heredia.
DELACHAUX, A. and TISSIERES, A. (1946): L'adaptation tissulaire à l'hypoxydose. Helv. med. Acta, 13: 333–338.
DUCKWORTH, M. W. (1961): Tissue changes accompanying acclimatization to low atmospheric oxygen in the rat. J. Physiol. (Lond.), 156: 603–610.
ESTABROOK, R. W. (1967): In Methods in Enzymology, Vol. X, Academic Press, New York, 575.
HAWKINS, S. F., HANKINSON, J. B., MERRIT, J. H. and MEDINA, M. A. (1971): Effect of reduced barometric pressure on drug action and metabolism in mice. Biochem. Pharmacol., 20: 2221–2229.
HOGEBOOM, G. H. (1955): In Methods in Enzymology, Vol. I, Academic Press, New York, 16.
JOHNSON, T. F. and GOLD, A. J. (1970): Effects of altitude exposure on cell metabolism. Fed. Proc., 29: 592.
LA DU, B. N., MANDEL, H. G. and WAY, E. L. (1971): In Fundamentals of Drug Metabolism and Drug Disposition. Williams and Wilkins, Baltimore, 575.
MEERSON, F. A. (1975): Role of synthesis of nucleic acids and protein in adaptation to the external environment. Physiol. Rev., 55: 79–123.
MENSEN DE SILVA, E. and CAZORLA, A. (1973): Lactate,α-GP, and Krebs cycle in sea-level and high altitude native guinea pigs. Amer. J. Physiol., 224: 669–672.
MORAN, O. (1978): Un modelo de hipoxia intermitente: estudio morfométrico. B.S. Thesis, Universidad Peruana Cayetano Heredia.
OU, L. C. and TENNEY, S. M. (1970): Properties of mitochondria from hearts of cattle acclimatized to high altitude. Resp. Physiol., 8: 151–159.
PHILLIPS, A. V. and LANGDON, R. G. (1962): Hepatic triphosphopyridine nucleotidecytochrome c reductase: Isolation, characterization and kinetic studies. J. biol. Chem., 237: 2652–2660.
REMMER, H., et al. (1966): Drug Interaction with hepatic microsomal cytochrome. Molec. Pharmacol., 2: 187–190.
ROMERO, G., NAVARRO, J., CAZORLA, A. and ALVAREZ, J. (1976): Ambient temperature and Hypoxia: A differential study of liver catalase and cytochrome oxidase in guinea pig. Johns Hopk. Med. J., 139: 82–86.
SCHNAKEMBERG, D. D. and HANNONG, J. P. (1970): Hypophagic responses of translocation to high altitude. Fed. Proc., 29: 592.
SHERTZER, H. G. and CASCARANO, J. (1972): Mitochondrial alterations in heart, liver and kidney of altitude-acclimated rats. Amer. J. Physiol., 223: 632–636.
STAUFFER, C. E. (1975): A linear standard curve for the Folin-Lowry determination of protein. Anal. Biochem., 69: 646–648.
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Rivera, K., Aguilar, R., Burgos, C. et al. The oxygen consuming systems of the liver of mice exposed to simulated high altitude. Int J Biometeorol 25, 293–297 (1981). https://doi.org/10.1007/BF02198244
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DOI: https://doi.org/10.1007/BF02198244