Summary
Young saplings of Pinus sylvestris were fumigated for 3 months with ammonia in concentrations ranging from 0 to 240 μg m-3. Despite the much higher concentrations than normal in the field, no visible damage occurred. Photosynthesis, dark respiration, transpiration and biomass production were stimulated. At 240 μg m-3 with high irradiance (PAR: 950 μmol m-2 s-1), net photosynthesis was stimulated by 24% and dark respiration by 76%. Intitial light use efficiency was not significantly affected. Transpiration increased, both in the dark and at 950 μmol m-2 s-1 by 40% and 57%, respectively. In the presence of ammonia, stomatal control was less efficient. Though growth of roots was not affected by NH3, that of current year needles was stimulated, resulting in an increased mass ratio of needles to roots. The nitrogen content of the needles increased, but the contents of other mineral components did not change significantly. Due to increased transpiration per unit of needle area and increased mass of needles per tree, water loss per tree was about twice as high in the treatment with 240 μg m-3 as in the control. Towards the end of fumigation, a 10-day period without water supply followed and then the water potential of the shoots was measured as an indicator of water demand. This demand was higher with higher concentrations of NH3, suggesting a higher risk of injury from drought.
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References
Aerts R, Berendse F (1988) The effect of increased nutrient availability on vegetation dynamics in wet heathlands. Vegetatio 76: 63–69
Aneja VP, Stahel E, Rogers HH, Witherspoon AM, Heck WW (1978) Calibration and performance of a thermal converter in continuous atmospheric monitoring of ammonia. Anal Chem 50: 1705–1708
Bacic T, Baas P, Van der Eerden LJ (1992) Needle wax surface structure of Pinus sylvestris L., as affected by ammonia. Acta Bot Neerl (in press)
Baule H, Fricker C (1970) The fertilizer treatment of forest trees. BLV, Munich
Berendse F, Aerts R (1987) Nitrogen-use-efficiency: a biologically meaningful definition? Funct Ecol 1: 293–296
Brix H (1981) Effects of nitrogen fertilizer source and application rates on foliar nitrogen concentration, photosynthesis and growth on Douglas fir. Can J For Res 4: 775–780
De Kam M (1990) (ed) The epidemiology of Sphearopsis sapinea; a cause of shoot and bark necrosis in the Netherlands (in Dutch) Res Inst For Urban Ecol (no. 598). Wageningen, The Netherlands
Dueck TA, Dorel F, Ter Horst R, Van der Eerden LJ (1991). Effects of ammonia and sulphur dioxide on the frost sensitivity of Pinus sylvestris. Water Air Soil Pollut 54: 35–49
Erisman J, Vermetten A, Pinksterboer E, Asman WAM, Ypelaan A, Slanina J (1987) Atmospheric ammonia: distribution, equilibrium with aerosols and conversion rate to ammonium. In: Asman WAM, Diederen S (eds) Ammonia and acidification. Proc Conf Bilthoven, RIVM, Bilthoven, The Netherlands, pp 59–71
Field C, Mooney HA (1986) The photosynthesis-nitrogen relationship in wild plants. In: Givnish TJ (eds) On the economy of plant form and function. Cambridge University Press, New York, pp 25–55
Goudriaan J, Van Laar HH (1978) Relations between leaf resistance CO2 concentration and CO2 assimilation in maize, beans, lanang grass and sunflower. Photosynthetica 12:241–249
Heij GT, Schneider T (eds) (1991) Acidification research in the Netherlands. (Environmental science series no. 46) Elsevier, Amsterdam
Hirose T, Werger MJA (1987). Nitrogen use efficiency in instantaneous and daily photosynthesis of leaves in the canopy of a Solidago altissima stand. Physiol Plant 70: 215–222
Hüttl RF (1990) Nutrient supply and fertilizer experiments in view of N saturation. Plant Soil 128: 45–58
Leverenz JW (1987) Chlorophyll content and the light response curve of shade-adapted conifer needles. Physiol Plant 71:20–29
Lindgren BW, McElrath GW (1969) Introduction to probability and statistics. McMillan, Toronto
Losada M, Arnon DJ (1963) Selective inhibitors. In: Hochster RM, Quastel JH (eds) Metabolic inhibitors ⊕ comprehensive treatise. V2, New York, pp 503–511
Malànchuk JL, Nilsson J (1989) The role of nitrogen in the acidification of soils and surface waters. Nordic Council of Ministers, Copenhagen, Denmark
Matyssek R, Schulze ED (1987) Heterosis in hybrid larch (Larix decidua X leptolepsis). I. The role of leaf characteristics. Trees 1: 219–224
Mitchel CA, Stocking CR (1975) Kinetics and energetics of light driven chloroplast glutamine synthesis. Plant Physiol 55: 59–63
Mohren FMJ (1987) Simulation of forest growth applied to Douglas fir stands in the Netherlands. Thesis, Agricultural University of Wageningen, The Netherlands
NG PAP, Jarvis PG (1980) Hysteresis in the response of stomatal conductance in Pinus sylvestris L. needles to light: observations and a hypothesis. Plant Cell Environ 3: 207–216
Nihlgard B (1985) The ammonium-hypothesis: an additional explanation to the forest dieback i Europe. Ambio 14: 1–8
Raven JA (1988) Acquisition of nitrogen by the shoots of land plants: its occurrence and implications for acid-base regulation. New Phytol 109:1–20
Reich PB, Walters MB, Tabone TJ (1989) Response of Ulmus americana seedlings to varying nitrogen and water status. 2. Water and nitrogen use efficiency in photosynthesis. Tree Physiol 5: 173–184
Roelofs JJGM, Kempers AJ, Houdijk ALFM, Jansen J (1985). The effects of airborne ammonium sulphate on Pinus nigra var. maritima in the Netherlands. Plant Soil 84: 45–56
Scholander PF, Hammel HT, Bradstreet EDS, Hemmingsen EA (1965) Sap pressure in vascular plants. Science 48: 339–446
Schulze ED, Lange OL, Oren R (eds) (1989) Forest decline and air pollution. (Ecological studies 77) Stringer, Berlin, Heidelberg New York
Skeffington RA, Wilson EJ (1988) Excess nitrogen deposition: issues for consideration. Environ Pollut 54: 161–162
Thijsse G, Baas P (1990) “Natural” and NH3-induced variation in epicuticular needle wax marphology of Pseudotsuga menziesii. Trees 4: 111–119
Van den Burg J (1990) Foliar analysis for determination of tree status: a compilation of literature data. Inst For Urban Ecol Rep no 591-II, Wageningen, The Netherlands
Van den Burg J, Kiewiet HP (1989) Livestock density and nutrient status of the needles of Scots Pine, Douglas Fir and Corsican Pine in the Peel region in the period 1956–1988 (in Dutch). Rep 559, Inst For Urban Ecol, Wageningen, The Netherlands
Van der Eerden LJ (1982) Toxicity of ammonia to plants. Agric Environ 7:223–235
Van der Eerden LJ, Dueck TA, Elderson J, Van Dobben HF, Berdowski JJM, Latuhihin M, Prins AH (1990) Effects of NH3 and (NH4)2SO4 deposition on terrestrial semi-natural vegetation on nutrient-poor soils. Res Inst Plant Prot, Rep R90/06, Wageningen, The Netherlands
Van der Eerden LJ, Lekkerkerk LJ, Smeulders SM, Jansen AE (1992) Effects of atmospheric ammonia and ammonium sulphate on Douglas fir (Pseudotsuga menziesii). Environ Pollut (in press)
Van der Eerden LJ, Dueck TA, Van Dobben HF, Berdowski JJM, Greven H, Van Dobben HF (1991) Influence of NH3 and (NH4)2SO4 deposition on heathland vegetation. Acta Bot Neerl 40: 281–296
Van Dijk HFG, Roelofs JGM (1988) Effects of excessive ammonium deposition on the nutritional status and condition of pine needles Physiol Plant 73: 494–501
Van Hove LWA, Adema EH, Vredenberg WJ, Pieters GA (1989a) A study of the absorption of NH3 and SO2 on leaf surfaces. Atmos Environ 23: 1479–1486
Van Hove LWA, Van Kooten O, Adema EH, Vredenberg WJ, Pieters GA (1989b) Physiological effects of long term exposure to low and moderate concentrations of atmospheric NH3 on poplar trees. Plant Cell Environ 12: 899–908
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Van der Eerden, L.J.M., Perez-Soba, M.G.F.J. Physiological responses of Pinus sylvestris to atmospheric ammonia. Trees 6, 48–53 (1992). https://doi.org/10.1007/BF00224499
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DOI: https://doi.org/10.1007/BF00224499