Summary
The Pelomyxa palustris amoebae used in this study were multinucleate, herbivorous protozoans. All nuclei within a single organism were similar, but several types of nuclei were seen in different amoeba. These nuclei might represent various stages of mitosis although metaphase and anaphase stages were never seen. Rod-shaped bacteria within vesicles characteristically surrounded the nuclei. Bacterial rods of this as well as another type also occurred within vesicles in the cytoplasm. The nuclear envelope contained annuli and it was covered externally by minute vesicles. Nucleoli and micronucleoli were most frequently located along the inner surface of the nuclear envelope. Clusters of electron-opaque spheroids were found within the nucleoli; sometimes, they existed free in the nucleoplasm. Intranuclear globules of lipidlike material were often seen.
Mitochondria, Golgi bodies, contactile vacuoles, and crystal vacuoles were definitely absent in P. palustris. The cytoplasm contained many food vacuoles and clear vacuoles of various sizes. Vacuole-like aggregations, probably containing glycogen, were present.
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References
Andresen, N., C. Chapman-Andresen, and J. R. Nilsson: The fine structure of Pelomyxa palustris. In: Progress in protozoology. Excerpt. Med. Fdn., Int. Congr. Ser. 91, 258 (1965).
Cohen, A. I.: Electron microscopic observations of Amoeba proteus in growth and inanition. J. biophys. biochem. Cytol. 3, 859–865 (1957).
Daniels, E. W.: Pelomyxa palustris from Illinois. Argonne National Laboratory Biological and Medical Research Div. Quart. Rpt. ANL-5655, 104–106 (1956).
—, and E. P. Breyer: Fine structure of the herbivorous giant amoeba, Pelomyxa palustris. Argonne National Laboratory Biological and Medical Research. Div. Annual Rpt. for 1965. ANL-7136, 216–220 (1965).
—, E. P. Breyer, and R. R. Kudo: fine structure of the giant, algae-eating amoeba, Pelomyxa palustris. Amer. Zool. 5, 734 (1965).
—, and L. E. Roth: Electron microscopy of mitosis in a radiosensitive giant amoeba. J. Cell Biol. 20, 75–84 (1964).
Deutsch, K., and V. Zaman: An electron microscopic study of Entamoeba invadens Rodhain 1934. Exp. Cell. Res. 17, 310–319 (1959)
Driml, M.: Electron and light microscope studies of Endamoeba terrapinae. Proc. Iowa Acad. Sci. 68, 581–585 (1961).
Esper, H.: Studies on the nucleolar vacuole in the oogenesis of Arbacia punctulata. Exp. Cell Res. 38, 85–96 (1965).
Greider, M. H., W. J. Kostir, and W. J. Frajola: Electron microscopy of Amoeba proteus. J. Protozool. 5, 139–146 (1958).
Kudo, R. R.: Pelomyxa palustris Greeff. I. Cultivation and general observations. J. Protozool. 4, 154–164 (1957).
—: Protozoology, 4th edit. Springfield (Ill.): Ch. C. Thomas 1954.
—: Pelomyxacarolinensis Wilson. II. Nuclear division and plasmotomy. J. Morph. 80, 93–144 (1947).
Leiner, M.: Das Glykogen in Pelomyxa palustris Greeff mit Beiträgen zur Kenntnis des Tieres. Arch. Protistenk. 47, 253–307 (1924).
—, u. M. Wohlfeil: Das Symbiontische Bakterium in Pelomyxa palustris Greeff III. Z. Morph. 42, 529–549 (1954).
— — u. D. Schmidt: Pelomyxa palustris Greeff. Ann. Sci. nat.Zool., Sér. XI, 537–594 (1954).
Mercer, E. H.: An electron microscopic study of Amoeba proteus. Proc. roy. Soc. B 150, 216–232 (1959).
Miller, J. H., J. C. Swartzwelder, and J. E. Deas: An electron microscopic study of Entamoeba histolytica. J. Parasit. 47, 577–587 (1961).
Okada, Y. K.: Transplantationsversuche an Protozoen. Arch. Protistenk. 69, 39–94 (1930).
Osada, M.: Electron-microscopic studies on protozoa. I. Fine structure of Entamoeba histolytica. Keio J. Med. 8, 99–103 (1959).
Pappas, G. D.: Electron microscope studies on amoebae. Ann. N.Y. Acad. Sci. 78, 448–473 (1959).
Revel, J. P.: Electron microscopy of glycogen. J. Histochem. Cytochem. 12, 104–114 (1964).
Roth, L. E., and E. W. Daniels: Infective organisms in the cytoplasm of Amoeba proteus. J. biophys. biochem. Cytol. 9, 317–323 (1961).
—, S. W. Obetz, and E. W. Daniels: Electron microscopic studies of mitosis in amoebae. I. Amoeba proteus. J. biophys. biochem. Cytol. 8, 207–220 (1960).
Siddiqui, W. A., and M. A. Rudzinska: The fine structure of axenically grown trophozoites of Entamoeba invadens with special reference to the nucleus and helical ribonucleoprotein bodies. J. Protozool. 12, 448–458 (1965).
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Work supported by the U. S. Atomic Energy Commission.
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Daniels, E.W., Breyer, E.P. & Kudo, R.R. Pelomyxa palustris Greeff. Z. Zellforsch 73, 367–383 (1966). https://doi.org/10.1007/BF00329017
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DOI: https://doi.org/10.1007/BF00329017