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Functional and structural interactions between osteoblastic and preosteoclastic cells in vitro

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Abstract

Osteoblasts are involved in the bone resorption process by regulating osteoclast maturation and activity. In order to elucidate the mechanisms underlying osteoblast/preosteoclast cell interactions, we developed an in vitro model of co-cultured human clonal cell lines of osteoclast precursors (FLG 29.1) and osteoblastic cells (Saos-2), and evaluated the migratory, adhesive, cytochemical, morphological, and biochemical properties of the co-cultured cells. In Boyden chemotactic chambers, FLG 29.1 cells exhibited a marked migratory response toward the Saos-2 cells. Moreover, they preferentially adhered to the osteoblastic monolayer. Direct co-culture of the two cell types induced: (1) positive staining for tartrate-resistant acid phosphatase in FLG 29.1 cells; (2) a decrease of the alkaline phosphatase activity expressed by Saos-2 cells; (3) the appearance of typical ultrastructural features of mature osteoclasts in FLG 29.1 cells; (4) the release into the culture medium of granulocyte-macrophage colony stimulating factor. The addition of parathyroid hormone to the co-culture further potentiated the differentiation of the preosteoclasts, the cells tending to fuse into large multinucleated elements. These in vitro interactions between osteoblasts and osteoclast precursors offer a new model for studying the mechanisms that control osteoclastogenesis in bone tissue.

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Authors and Affiliations

  1. Department of Human Anatomy and Histology, University of Florence, Medical School, Viale Morgagni 85, I-50139, Florence, Italy

    Sandra Zecchi Orlandini & Lucia Formigli

  2. Department of Clinical Physiopathology, University of Florence, Medical School, Viale Pieraccini 6, I-50139, Florence, Italy

    Susanna Benvenuti, Laura Lasagni, Laura Masi & Maria Luisa Brandi

  3. Institute of Pathological Anatomy, University of Florence, Medical School, Viale Morgagni 85, I-50139, Florence, Italy

    Alessandro Franchi

  4. U.O. Hematology, USL 10/D, Florence, Italy

    Pietro Antonio Bernabei & Valeria Santini

Authors
  1. Sandra Zecchi Orlandini
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  2. Lucia Formigli
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  3. Susanna Benvenuti
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  4. Laura Lasagni
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  5. Alessandro Franchi
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  6. Laura Masi
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  7. Pietro Antonio Bernabei
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  8. Valeria Santini
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  9. Maria Luisa Brandi
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Orlandini, S.Z., Formigli, L., Benvenuti, S. et al. Functional and structural interactions between osteoblastic and preosteoclastic cells in vitro. Cell Tissue Res 281, 33–42 (1995). https://doi.org/10.1007/BF00307956

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  • DOI: https://doi.org/10.1007/BF00307956

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