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Immunocytochemical localization of seminal proteins in salivary and lacrimal glands of the rat

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Abstract

Antibodies against 10 different secretory proteins from the accessory sex glands of the male rat were used for immunohistochemical studies of salivary and lacrimal glands from intact and castrated rats, at the light- and electron-microscopic levels. In the parotid gland, secretory acinar cells showed immunoreactivity with antibodies against prostatic binding protein, cystatin-related peptide and acid phosphatase (isoenzyme pI 8.0; 5.6) typical of ventral prostate, and seminal vesicle secretion VI. Western blotting analysis indicated that immunoreactivity against prostatic binding protein was attributable to a subunit, presumably C3. Acid phosphatase pI 5.6 showed a molecular weight of 66 kDa, which is at variance with the prostatic form. Immunoreactivity for secretory transglutaminase, derived from the coagulating gland, was restricted to myoepithelial and stromal cells. In castrated animals, the immunoreactivity of acinar cells was reduced to the background level, whereas stromal transglutaminase immunoreactivity was unaltered. The distribution pattern of immunoreactivity for the proteins mentioned was almost identical in the lacrimal gland. Significant differences were however observed in the immunoreactivity of the inframandibular gland, where serous glandular cells were non-immunoreactive for seminal proteins, with the exception of acid phosphatase isoenzyme pI 8.0. Granules present in the convoluted granular ducts were immunoreactive particularly for acid phosphatase (isoenzyme pI 5.6)but much less for cystatin-related peptide; immunoreactivity was reduced after castration. The straight portion of the inframandibular duct system was immunoreactive for transglutaminase, but no influence of castration was visible. The distribution of immunoreactivity for seminal proteins present in the salivary and lacrimal glands and the pronounced androgen-dependence of their expression point to functional relationships of the respective proteins at both glandular sites.

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  1. Eric A. Arce

    Present address: Department of Anatomy, P.O. Box 10008, 43699-0008, Toledo, OH, USA

Authors and Affiliations

  1. Institut für Anatomie und Zellbiologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, D-35033, Marburg, Germany

    G. Aumüller, Eric A. Arce, I. Dammshäuser & J. Seitz

  2. Laboratorium voor Experimentele Geneeskunde en Endocrinologie, Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium

    W. Heyns & I. Vercaeren

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  1. G. Aumüller
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  2. Eric A. Arce
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Aumüller, G., Arce, E.A., Heyns, W. et al. Immunocytochemical localization of seminal proteins in salivary and lacrimal glands of the rat. Cell Tissue Res 280, 171–181 (1995). https://doi.org/10.1007/BF00304522

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