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Immunohistochemical localization of opioid peptides in the brain of the leech Theromyzon tessulatum

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Summary

By use of antisera directed against met-enkephalin, leu-enkephalin, dynorphin or α-neoendorphin, immunoreactive structures were visualized in the central nervous system and proboscis of the leech Theromyzon tessulatum. Their distribution in the various compartments of the supra- and subesophageal ganglia was mapped. No correspondence could be established between the neurons containing met- or leu-enkephalin-like substances and the different types of neurosecretory cells classically described in Hirudinea. Successive localization of leu- and met-enkephalin on the same section revealed that these two peptides occur in different neurons. Only one cell located in compartment 6 of the supraesophageal ganglion was both dynorphin- and leu-enkephalin-positive. The other dynorphinimmunoreactive cells were not stained with the anti-leuenkephalin serum. The α-neoendorphin-immunopositive cells were leu-enkephalin immunonegative and vice versa.

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Authors and Affiliations

  1. Unité Associée au C.N.R.S. n∘ 148, Endocrinologie des Invertébrés, Université de Lille Flandres-Artois, Villeneuve-d'Ascq, France

    M. Verger-Bocquet & J. Malecha

  2. Unité 156, I.N.S.E.R.M., Lille, France

    G. Tramu

Authors
  1. M. Verger-Bocquet
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  2. J. Malecha
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  3. G. Tramu
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Verger-Bocquet, M., Malecha, J. & Tramu, G. Immunohistochemical localization of opioid peptides in the brain of the leech Theromyzon tessulatum . Cell Tissue Res. 250, 63–71 (1987). https://doi.org/10.1007/BF00214655

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  • DOI: https://doi.org/10.1007/BF00214655

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