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The effect of cytochalasin B on the enucleation of erythroid cells in vitro

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Summary

The process of nuclear extrusion continues when erythroid cells of mouse spleen are placed in vitro, although the process may be considerably retarded. This allows visualization of unusually large numbers of enucleating cells after 30 min in vitro. With this system, the mechanism of enucleation can be analyzed both qualitatively and quantitatively. We have used this system to study the effects of cytochalasin B and have found it to be a potent inhibitor of enucleation. These results suggest that microfilaments are involved in the process of enucleation.

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References

  • Andrew W (1965) Comparative hematology. Grune and Stratton, New York, p 145

    Google Scholar 

  • Arnold JM (1969) Cleavage furrow formation in a telolecithal egg (Loligo pealii), I. Filaments in early furrow formation. J Cell Biol 41:894–904

    Google Scholar 

  • Awai M, Okada S, Takebayashi J, Kubo T, Inoue M, Seno S (1968) Studies on the mechanism of denucleation of the erythroblast. Acta Haematol 39:193–202

    Google Scholar 

  • Bessis M (1973) Living blood cells and their ultrastructure. Springer-Verlag, New York, Chapter 2

    Google Scholar 

  • Bessis M, Bricka M (1952) Ect dynamique des cellules du sang. Son étude par la microcinematographie en contraste de phase Rev Hematol 7:407–435

    Google Scholar 

  • Campbell FR (1968) Nuclear elimination from the normoblast of fetal guinea pig liver as studied with electron microscopy and serial sectioning techniques. Anat Rec 160:539–543

    Google Scholar 

  • Carter SB (1967) Effects of cytochalasins on mammalian cells. Nature 213:261–264

    Google Scholar 

  • Jones OP (1960) Electron microscopic studies of the nuclear membrane in mitotic erythroblasts. Proc. VIIIth International Cong Hematology, Tokyo, Pan Pacific Press (Abstr.)

    Google Scholar 

  • Lin S, Lin DC, Flanagan MD (1978) Specificity of the effects of cytochalasin B on transport and motile processes. Proc Natl Acad Sci USA 75:329–333

    Google Scholar 

  • Lin DC, Tobin KD, Grumet M, Lin S (1980) Cytochalasins inhibit nuclei-induced actin polymerization by blocking filament elongation. J Cell Biol 84:455–460

    Google Scholar 

  • Orlic D, Gordon AS, Rhodin JAG (1965) An ultrastructural study of erythropoietin-induced red cell formation in mouse spleen. J Ultrastruct Res 13:516–542

    Google Scholar 

  • Poste G, Lyon NC (1978) Enucleation of cultured animal cells by cytochalasin B. In: Tanenbaum SW (ed) Cytochalasins, biochemical and cell biological aspects. North-Holland Publishing Co. Amsterdam, p 161

    Google Scholar 

  • Repasky EA, Eckert BS (1981a) A reevaluation of the process of enucleation in mammalian erythroid cells. In: Brewer G (ed) The red cell, 5th Ann Arbor Conference, Vol. 55, Progress in Clinical and Biological Research. AR Liss Co, New York, p 679

    Google Scholar 

  • Repasky EA, Eckert BS (1981b) Microtubules in mammalian erythroblasts. Are marginal bands present? Anat Embryol 162:419–424

    Google Scholar 

  • Schroeder TE (1968) Cytokinesis: Filaments in the cleavage furrow. Exp Cell Res 53:272–318

    Google Scholar 

  • Schroeder TE (1978) Cytochalasin B, cytokinesis, and the contractile ring. In: Tanenbaum SW (ed) Cytochalasins, biochemical and cell biological aspects. North-Holland Publishing Co. Amsterdam, p 91

    Google Scholar 

  • Seki M, Yoneyama T, Shirasawa H (1965) Role of the reticular cells during maturation process of the erythroblast. Report I. Denucleation of erythroblast by reticular cell. Electron microscopic study. Acta Pathol Jpn. 15:295–320

    Google Scholar 

  • Skutelsky E, Danon D (1967) An electron microscopic study of nuclear elimination from the late erythroblast. J Cell Biol 33:625–635

    Google Scholar 

  • Skutelsky E, Danon D (1970) Comparative study of nuclear expulsion from the late erythroblast and cytokinesis. Exp Cell Res 60:427–436

    Google Scholar 

  • Spudich JA, Lin S (1972) Cytochalasin B, its interation with actin and actinomyosin from muscle. Proc Natl Acad Sci USA 69:442–446

    Google Scholar 

  • Tavassoli M (1980) Bone marrow: The seedbed of blood. In: Wintrobe MM (ed) Blood, Pure and eloquent. McGraw-Hill, New York, p 57

    Google Scholar 

  • Tavassoli M, Crosby WH (1973) Fate of the nucleus of marrow erythroblasts. Science 173:912

    Google Scholar 

  • Tsiftsoglou AS, Barnett RJ, Sartorelli AC (1979) Enucleation of differentiated murine erythroleukemia cells in culture. Proc Natl Acad Sci USA 76:6381–6385

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomical Sciences, State University of New York at Buffalo, Buffalo, New York, USA

    Elizabeth A. Repasky & Barry S. Eckert

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  1. Elizabeth A. Repasky
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  2. Barry S. Eckert
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Supported by NSF Grant PCM 78-00573

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Repasky, E.A., Eckert, B.S. The effect of cytochalasin B on the enucleation of erythroid cells in vitro. Cell Tissue Res. 221, 85–91 (1981). https://doi.org/10.1007/BF00216572

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  • DOI: https://doi.org/10.1007/BF00216572

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