Summary
Correlative light-, fluorescence- and electron-microscopic examinations of catecholamine-storing cells in the adrenal medulla of the domestic fowl present unequivocal criteria to identify two types of avian adrenomedullary cells. A modified Karnovsky's fixative containing s-collidine was found to be a reliable prefixative to obtain a characteristic feature of the secretory granules in adrenaline- and noradrenaline-storing cells consistently.
Similar content being viewed by others
References
Benchimol S, Cantin M (1977) Ultrastructural cytochemistry of the human adrenal medulla. Histochem 54:9–26
Benedeczky I, Smith AD (1972) Ultrastructural studies on the adrenal medulla of golden hamster; origin and fate of secretory granules. Z Zellforsch 124:367–386
Coupland RE (1971) Observations on the form and size distribution of chromaffin granules and on the identity of adrenaline- and noradrenaline-storing chromaffin cells in vertebrates and man. Mem Soc Endocr 19:611–635
Coupland RE, Hopwood D (1966) The mechanism of the differential staining reaction for adrenaline and noradrenaline-storing granules in tissues fixed in glutaraldehyde. J Anat 100:227–243
Coupland RE, Pyper AS, Hopwood D (1964) A method for differentiating between noradrenaline- and adrenaline-storing cells in the light and electron microscope. Nature (Lond) 201:1240–1242
Eränkö O (1955) Histochemistry of noradrenaline in the adrenal medulla of rats and mice. Endocrinol 57:363–368
Grynszpan-Winograd O (1971) Morphological aspects of exocytosis in the adrenal medulla. Phil Trans Roy Soc Lond B 261:291–292
Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27:137A-138A
Kataoka K, Shimizu K, Yamamoto T, Ochi J (1979) Monoamine-containing cells in the gastric mucosa of the kitten; a new method employing comparative fluorescence and electron microscopy on identical cells. Acta Histochem Cytochem 12:383–390
Kobayashi S, Serizawa Y (1979) Stress-induced degranulation accompanied by vesicle formation in the adrenal chromaffin cells of the mouse. Arch Histol Jpn 42:375–388
Kobayashi S, Watanabe Y (1971) Selective staining of A cells in the freeze-dried and formaldehyde-fixed adrenal medulla with toluidine blue and pseudoisocyanin. Arch Histol Jpn 33:59–66
Kobayashi S, Serizawa Y, Fujita T, Coupland RE (1978) SGC (small granule chromaffin) cells in the mouse adrenal medulla; light and electron microscopic identification using semi-thin and ultra-thin sections. Endocrinol Jpn 25:467–476
Luft JH, Wood RL (1963) The extraction of tissue protein during and after fixation with osmium tetroxide in various buffer systems. J Cell Biol 19:46A
Sato T (1968) A modified method for lead staining of thin sections. J Electron Microsc 17:158–159
Takaya K (1970) Two types of chromaffin cells in the adrenals of the bullfrog, Rana catesbiana; a comparative study. Arch Histol Jpn 32:69–80
Tramezzani JH, Chiocchio S, Wassermann GF (1964) A technique for light and electron-microscopic identification of adrenalin- and noradrenalin-storing cells. J Histochem Cytochem 12:890–899
Tranzer J-P, Richards JG (1976) Ultrastructural cytochemistry of biogenic amines in nervous tissue; methodologic improvements. J Histochem Cytochem 24:1178–1193
Unsicker K (1973) Fine structure and innervation of the avian adrenal gland. I. Fine structure of adrenal chromaffin cells and ganglion cells. Z Zellforsch 145:389–416
Wood JG, Seelig LL, Benjamin CP (1971) Cytochemistry of epinephrine and norepinephrine adrenomedullary cells. Histochem 28:183–197
Author information
Authors and Affiliations
Additional information
This paper, in a more detailed form, will be submitted by the first author (N.C.) in partial fulfillment of the requirement of the degree of Doctor of Philosophy in the Agricultural Sciences
Predoctoral student supported by Ministry of Education, Science and Culture, Japan (Scholarship no. 15323), on leave from Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Kasetsart University, Thailand. One of the authors (N.C.) is very grateful to Emeritus Professor Dr. M. Yasuda for his kindness of offering an opportunity to stay at his department and for his encouragement and valuable advice during the course of the experiment
The authors are indebted to Associate Professor Dr. T. Watanabe for his evaluation and criticism of the manuscript and also to Dr. K. Hirunagi, Mr. Y. Ohmori and Mr. H. Chikazawa for their skillful technical assistance
Rights and permissions
About this article
Cite this article
Chungsamarnyart, N., Fujioka, T. & Kitoh, J. Correlative light-, fluorescence- and electron-microscopic studies of catecholamine-storing cells in the adrenal medulla of the domestic fowl. Cell Tissue Res. 217, 471–477 (1981). https://doi.org/10.1007/BF00219358
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00219358