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LH-producing cells in the ovine pituitary

An electron microscopic immunocytochemical study

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Summary

An immunocytochemical technique using the peroxidase-antiperoxidase complex (PAP) was applied to identify and characterize the LH-secreting cells in the ovine pituitary at the ultrastructural level. These cells, round or oval in shape, possessing flattened cisternae of the rough endoplasmic reticulum, contain one class of secretory granules (mean diameter 250 nm) and large dense bodies (600 to 800 nm in diameter). LH molecules and the two subunits LHα and LHβ were localized on the secretory granules and on the small granules near the Golgi complex. The large dense bodies, the cisternae of the endoplasmic reticulum and the saccules of the Golgi complex showed no reaction product.

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Abbreviations

O-LH:

ovine luteinizing hormone

b-LH:

bovine luteinizing hormone

p-LH:

porcine luteinizing hormone

p-LHα:

porcine LHα subunit

p-LHβ :

porcine LHβ subunit

O-FSH:

ovine follicle stimulating hormone

b-TSH:

bovine thyrotropic hormone

A-b LH:

antiserum to bovine LH

A-pLHα:

antiserum to porcine LHα subunit

A-pLHβ :

antiserum to porcine LHβ subunit

References

  • Barnes, B.G.: Electron microscope studies on the secretory cytology of the mouse anterior pituitary. Endocrinol. 71, 618–628 (1962)

    Google Scholar 

  • Barnes, B.G.: The fine structure of the mouse adenohypophysis in various physiological states. In: Cytologie de l'adénohypophyse (J. Benoit and Da Lage, ed.). Paris: Editions du Centre National de la Recherche Scientifique 1963

    Google Scholar 

  • Beauvillain, J.C., Mazzuca, M., Dubois, M.P.: The thyrotropic cells of the guinea pig pituitary. Electron microscopic study after characterization by immunocytochemical means. Cell Tiss. Res. 174, 233–244 (1976)

    Google Scholar 

  • Beauvillain, J.C., Tramu, G., Dubois, M.P.: Characterization by different techniques of adrenocorticotropin and gonadotropin producing cells in lerot pituitary (Eliomys quercinus). Cell Tiss. Res. 158, 301–317 (1975)

    Google Scholar 

  • Dacheux, F., Dubois, M.P.: Ultrastructural localization of prolactin growth hormone and luteinizing hormone by immunocytochemical techniques in the bovine pituitary. Cell Tiss. Res. 174, 245–260 (1976 a)

    Google Scholar 

  • Dacheux, F., Dubois, M.P.: Immunocytologie ultrastructurale des cellules à prolactine, et des cellules somatotropes et gonadotropes de l'hypophyse de bovins. J. Physiol. (Lond.) 72, (5), 38A (1976b)

  • Dacheux, F., Dubois, M.P.: Etude comparée de l'immunoréactivité des cellules à prolactine, et des cellules gonadotropes LH de l'hypophyse de béliers et de bovins. J. Physiol. (Lond.) 72, (6), 9B (1976c)

  • Dubois, M.P.: Les cellules à hormones glycoprotidiques du lobe antérieur de l'hypophyse: séparation par immunofluorescence des cellules thyréotropes et des cellules gonadotropes dans l'hypophyse des bovins, ovins, porcins. Ann. Recher. Veter. 2, 197–222 (1971)

    Google Scholar 

  • Dubois, M.P.: Localisation par immunocytologie des hormones glycoproteïques hypophysaires chez les Vertébrés. Coll. “Hormones glycoprotéïques hypophysaires”. I.N.S.E.R.M. 9, 27–47 (1972)

    Google Scholar 

  • Dubois, P.M., Dubois, M.P.: Mise en évidence par immunofluorescence de l'activité gonadotrope LH dans l'antéhypophyse foetale humaine. Coll. “Sexual Endocrinology of the perinatal period”, Lyon (M.F. Forest and J. Bertrand, eds.) I.N.S.E.R.M. 32, 37–61 (1974)

  • Farquhar, M.G., Rinehart, J.F.: Electron microscopic studies of the anterior pituitary gland of castrated rats. Endocrinol. 54, 516–541 (1954)

    Google Scholar 

  • Girod, C.: Recherche sur les cellules gonadotropes antéhypophysaires du Hérisson: étude cytologique, ultrastructurale et cytophysiologique. In: Fonction gonadotrope et rapport hypothalamohypophysaire chez les animaux sauvages (M. Herlant, ed.). Paris: Masson et Cie. 1971

    Google Scholar 

  • Herlant, M.: Ultrastructure des cellules gonadotropes de l'hypophyse chez les Mammifères. In: Hormones glycoprotéïques hypophysaires (M. Jutisz, ed.), pp. 5–29. Paris: Editors I.N.S.E.R.M. 1972

    Google Scholar 

  • Herlant, M., Ectors, F.: Les cellules gonadotropes de l'hypophyse chez le porc. Z. Zellforsch. 101, 212–231 (1969)

    Google Scholar 

  • Hutson, J.C., Gardner, P.J., Bauer, T.W., Moriarty, G.C.: Technical considerations in immunocytochemistry. Anat. Rec. 184, 433–434 (1976)

    Google Scholar 

  • Kurosumi, K., Oota, Y.: Electron microscopy of two types of gonadotrophs in the anterior pituitary gland of persistent oestrus and persistent dioestrus rats. Z. Zellforsch 85, 34–46 (1968)

    Google Scholar 

  • Li, J.Y., Dubois, M.P., Dubois, P.M.: Somatotrophs in the human fetal anterior pituitary. An electron microscopic-immunocytochemical study. Cell Tiss. Res. 181, 545–552 (1977)

    Google Scholar 

  • Mikami, S., Daimon, T.: Cytological and cytochemical investigations of the adenohypophysis of the sheep. Arch. Histol. Japon. 29, (5), 427–445 (1968)

    Google Scholar 

  • Moriarty, G.C.: Adenohypophysis: ultrastructural cytochemistry. A review. J. Histochem. Cytochem. 21, 855–894 (1973 b)

    Google Scholar 

  • Moriarty, G.C.: Electron microscopic-immunocytochemical studies of rat pituitary gonadotrophs: a sex difference in morphology and cytochemistry of LH cells Endocrinol. 97, 1215–1225 (1975)

    Google Scholar 

  • Moriarty, G.C.: Immunocytochemistry of the pituitary glycoprotein hormones. J. Histochem. Cytochem. 24, (7), 846–863 (1976)

    Google Scholar 

  • Moriarty, G.C., Garner, L.L.: Immunocytochemical studies of cells in the rat adenohypophysis containing both ACTH and FSH. Nature (Lond.) 265, 5592, 356–358 (1977)

    Google Scholar 

  • Moriarty, G.C., Halmi, N.S.: Electron microscopic study of the adrenocorticotropin producing cell with the use of unlabeled antibody and the soluble peroxidase-antiperoxidase complex. J. Histochem. Cytochem. 20, 590–603 (1972 a)

    Google Scholar 

  • Moriarty, G.C., Halmi, N.S.: Adrenocorticotropin production by the intermediate lobe of the rat pituitary. An electron microscopic-immunohistochemical study. Z. Zellforsch. 132, 1–14 (1972 b)

    Google Scholar 

  • Moriarty, G.C., Moriarty, C.M., Sternberger, L.A.: Ultrastructural immunocytochemistry with unlabeled antibodies and the peroxidase-antiperoxidase complex. A technique more sensitive than radioimmunossay. J. Histochem. Cytochem. 21, 825–833 (1973 a)

    Google Scholar 

  • Moriarty, G.C., Tobin, R.B.: Ultrastructural immunocytochemical characterization of the thyrotroph in rat and human pituitaries. J. Histochem. Cytochem. 24 (11), 1131–1139 (1976)

    Google Scholar 

  • Nakane, P.K.: Classifications of anterior pituitary cell types with immunoenzyme histochemistry. J. Histochem. Cytochem. 18, 9–20 (1970)

    Google Scholar 

  • Nakane, P.K.: Application of peroxidase-labeled antibodies to the intracellular localization of hormones. Acts endocr. (Kbh). (Suppl.) 153, 190–202 (1971)

    Google Scholar 

  • Nakane, P.K.: Identification of anterior pituitary cells by immunoelectron microscopy. In: The anterior pituitary (A. Tixier-Vidal and M.G. Farquhar eds.), pp. 45–61. New York: Acad. Press 1975

    Google Scholar 

  • Pelletier, G., Leclerc, R. Labrie, F.: Identification of gonadotropic cells in the human pituitary by immunoperoxidase technique. Molec. Cell. Endocrinol. 6, 123–126 (1976)

    Google Scholar 

  • Petrali, J.P., Hinton, D.M., Moriarty, G.C., Sternberger, L.A.: The unlabeled antibody enzyme method of immunocytochemistry. Quantitative comparison of sensitivities with and without peroxidaseantiperoxidase complex. J. Histochem. Cytochem. 22, 782–801 (1974)

    Google Scholar 

  • Stefanini, M., Martino, C. de, Zamboni, L.: Fixation of ejaculated spermatozoa for an electron microscopy. Nature (Lond.) 216, 173–174 (1967)

    Google Scholar 

  • Sternberger, L.A. Hardy, P.H., JR., Cuculis, J.J., Meyer, H.G.: The unlabeled antibody enzyme method of immuno-histochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antiperoxidase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18, 315–333 (1970)

    Google Scholar 

  • Tixier-Vidal, A.: Ultrastructure of anterior pituitary cells in culture. In: The anterior pituitary (A. Tixier-Vidal and M.G. Farquhar, ed.), pp. 181–229. New York: Acad. Press 1975 b

    Google Scholar 

  • Tixier-Vidal, A., Kerdelhue, B., Berault, B., Picart, R., Justisz, M.: Action, in vitro du facteur hypothalamique de libération de l'hormone luteinisante (LRF) sur l'antéhypophyse d'agnelle. Etude ultrastructurale des tissues incubés. J. comp. Endocrinol. 17, 33–59 (1971)

    Google Scholar 

  • Tixier-Vidal, A., Tougard, C., Kerdelhue, B., Jutisz, M.: Light and electron microscopic studies on immunocytochemical localization or gonadotropic hormones in the rat pituitary gland with antisera against ovine FSH, LH, LHα and LHβ. Ann. N.Y. Acad. Sci. 254, 433–461 (1975 a)

    Google Scholar 

  • Tixier-Vidal, A., Tougard, C., Picart, R.: Subcellular localization of some protein and glycoprotein hormones of the hypothalamus hypophyseal axis as revealed by the peroxidase-labeled antibody method. Symposium “Immunoenzymatic techniques”. I.N.S.E.R.M., Feldmann et al., ed., 307–321 (1976)

  • Tougard, C., Kerdelhue, B., Tixier-Vidal, A., Jutisz, M.: Light and electron microscope localization of binding sites of antibodies against ovine luteinizing hormone and its two subunits in rat adenohypophysis using peroxidase labeled antibody technique. J. Cell Biol. 58, 503–521 (1973)

    Google Scholar 

  • Yoshimura, F., Harumiya, K.: Electron microscopy of anterior lobe of pituitary in normal and castrated rats. Endocr. jap. 12, 119–152 (1965)

    Google Scholar 

  • Zamboni, L., Martino, C. de,: Buffered picric acid formaldehyde: a new rapid fixation for electron microscopy. J. Cell Biol. 35, 148A (1967)

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Authors and Affiliations

  1. Laboratoire de Physiologie Comparée, Faculté des Sciences, Tours, France

    F. Dacheux

  2. I.N.R.A. — Physiologie de la Reproduction, Nouzilly, France

    M. P. Dubois

Authors
  1. F. Dacheux
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  2. M. P. Dubois
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Dacheux, F., Dubois, M.P. LH-producing cells in the ovine pituitary. Cell Tissue Res. 188, 449–463 (1978). https://doi.org/10.1007/BF00219783

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