Summary
The effects of penicillin and chlortetracyline HCl on the fine structure of the intracellular symbiotes of the pea aphid were studied in an attempt to remove the symbiote population. High penicillin concentrations, 1% and 0.1%, caused symbiote breakdown but were toxic and/or repellent to the aphids; at 0.1% specific effects were observed on the symbiotes' cell walls. After the use of 0.01% penicillin in the aphid diet, the symbiotes had abnormal cell walls and were abnormally dilated; however, symbiote division and transmission from one aphid generation to the next seemed unaffected and the aphids appeared normal. Aphids fed 0.1% chlortetracycline failed to reproduce. After 7 days, their symbiotes were found to break down at a high rate but aphid mitochondria were also adversely affected at this stage. Following 0.002% chlortetracycline, the aphids produced aposymbiotic progeny with apparently normal mitochondrial populations; these larvae failed to develop.
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Akey, D. H., Beck, S. D.: Continuous rearing of the pea aphid, Acyrthosiphon pisum, on a holidic diet. Ann. entomol. Soc. Amer. 64, 353–356 (1971)
Auclair, J. L.: Nutrition of plant-sucking insects on chemically defined diets. Entomologia. exp. appl. 13, 307–319 (1969)
Buchner, P.: Endosymbioses of animals with plant microorganisms. New York: Interscience 1965
Erhardt, P.: Entwicklung und Symbionten geflügelter und ungeflügelter Virgines von Aphis fabae Scop. unter dem Einfluß künstlicher Ernährung. Z. Morph. Ökol. Tiere 57, 295–319 (1966a)
Erhardt, P.: Die Wirkung von Lysozyminjectionen auf Aphiden und deren Symbionten. Z. vergl. Physiol. 53, 310–141 (1966b)
Erhardt, P.: Die Wirkung verschiedener Spurenelemente auf Wachstum, Reproduktion und Symbionten von Neomyzus circumflexus Buckt (Aphididae, Homoptera, Insecta), bei künstlicher Ernährung. Z. vergl. Physiol. 58, 47–75 (1968)
Erhardt, P.: Die Rolle von Methionin, Cystein, Cystin und Sulfat bei der künstlichen Ernährung von Neomyzus (Aulacortum) circumflexus Buckt (Aphididae, Homoptera, Insect). Biol. Zbl. 88, 335–348 (1969)
Erhardt, P., Schmutterer, H.: Die Wirkung verschiedener Antibiotica auf Entwicklung und Symbionten künstlich ernährter Bohnenblattläuse (Aphis fabae Scop.). Z. Morph. Ökol. Tiere 56, 1–20 (1966)
Firz-James, P., Hancock, P.: The initial structure lesion of penicillin action on Bacillus megaterium. J. Cell Biol. 26, 657–667 (1965)
Flynn, E. H., Godzeski, C. W.: Penicillin and cephalosporins. In: The antibiotics (eds.: Gottlieb, D., Shaw, P. D.), vol. 1, p. 1–19. Berlin-Heidelberg-New York: Springer 1967
Griffiths, G. W., Beck, S. D.: Intracellular symbiotes of the pea aphid, Acyrthosiphon pisum. J. Insect Physiol. 19, 75–84 (1973)
Hinde, R.: Structural and physiological studies of the mycetome symbiotes of aphids. Ph. D. Thesis, Universtiy of Sydney (1970)
Hinde, R.: The control of the mycetome symbiotes of the aphids, Brevicoryne brassicae, Myzus persicae and Macrosiphum rosae. J. Insect Physiol. 17, 1791–1800 (1971a)
Hinde, R.: The fine structure of the mycetome symbiotes of the aphids, Brevicoryne brassicae, Myzus persicae, and Macrosiphum rosae. J. Insect Physiol. 17, 2035–2050 (1971b)
Lamb, K. P., Hinde, R.: Structure and development of the mycetome in the cabbage aphid, Brevicoryne brassicae. J. Insect. Pathol. 9, 3–11 (1967)
Lanham, U. N.: The Blockman Bodies: hereditary intracellular symbionts of insects. Biol. Rev. 43, 269–286 (1968)
Laskin, A. I.: Tetracyclines. In: The Antibiotics (eds.: Gottlieb, D. Shaw, P. D.), vol. 1, p. 331–359. Berlin-Heidelberg-New York: Springer 1967
McLean, D. L., Houk, E. J.: Phase contrast and electron microscopy of the mycetocytes and symbiotes of the pea aphid, Acyrthosiphon pisum. J. Insect Physiol. 19, 625–635 (1973)
Milne, R. G., De Zoeten, G. A.: A comparison of some methods of preparation of thin sections of virus infected leaves for electron microscopy. J. Ultrastruct. Res. 19, 398–407 (1967)
Mittler, T. E.: Some effects on the aphid Myzus persicae of ingesting antibiotics incorporated into artificial diets. J. Insect Physiol. 17, 1333–1347 (1971)
Palade, G. E.: A study of fixation for electron microscopy. J. exp. Med. 95, 285–298 (1952)
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)
Stanier, R. Y., Doudoroff, M., Adelberg, E. A.: The microbial world, 3rd ed. New Jersey: Prentice-Hall Inc 1970
Uichanco, L. B.: Studies on the embryogeny and postnatal development of the Aphididae with special reference to the history of the “symbiotic organ” or “mycetom”. Phillip J. Sci. 24, 143–247 (1924)
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Research supported by the College of Agricultural and Life Sciences, University of Wisconsin, and by a research grant (GB 31904 X) from the National Science Foundation.
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Griffiths, G.W., Beck, S.D. Effects of antibiotics on intracellular symbiotes in the pea aphid, Acyrthosiphon pisum . Cell Tissue Res. 148, 287–300 (1974). https://doi.org/10.1007/BF00224257
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DOI: https://doi.org/10.1007/BF00224257